Centralized in vitro embryo production after ultrasound guided bovine oocyte collection: Effects of parity and superovulation treatment

The reproductive performance of cloned cattle was investigated by assessing the efficiency of transvaginal ultrasound-guided ovum pickup (OPU) and embryo production in vitro. Fetal fibroblasts from the endangered species, German Blackpied Cattle, had been used for nuclear transfer to produce three live cloned offspring (Lucas-Hahn et al. 2002 Theriogenology 57, 433). In the three cloned animals at 12–20 months of age, OPU was performed once per week and the total number of collected oocytes was recorded. In the case of Blondie, the procedure was terminated due to too small ovaries associated with insufficient function. Oocytes suitable for IVF were matured in vitro for 24 h and fertilized in vitro with the semen of a fertile bull. Oocytes derived from abbatoir ovaries were processed in parallel as controls. Embryos were in vitro-cultured in SOFaaBSA medium. Cleavage and developmental rates up to the morula/blastocyst stage were recorded in all groups. Statistical significance was tested using ANOVA and the Student-Newman-Keuls test. The results are presented in Table 1. Embryos from clones had lower cleavage and blastocyst rates compared to those derived from abattoir oocytes. However, results may have been confounded by potential OPU effects. Some of the blastocysts produced from Blacky (n = 5) and Paula (n = 2) were transferred to recipients. Two pregnancies resulted from the Paula transfers. The two male calves were delivered normally. After the completion of this experiment, all three cloned animals were artificially inseminated, became pregnant, delivered healthy calves, and are pregnant again at present. Further studies are needed to explore the fertility of cattle derived from somatic cloning. Table 1. OPU and in vitro embryo production in cloned cattle

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106 COMPARISON OF IN VITRO EMBRYO PRODUCTION IN PANAMA USING HOLSTEIN OOCYTES WITH BOS INDICUS SEXED SEMEN FROM DONORS IN DIFFERENT LOCATIONS: FIELD DATA

Reproduction Fertility and Development ◽

10.1071/rdv28n2ab106 ◽

2016 ◽

Vol 28 (2) ◽

pp. 183

Author(s):

S. J. R. Rodriguez ◽

Y. E. Ramirez ◽

E. Gomes ◽

L. F. Nasser ◽

J. H. F. Pontes ◽

...

Keyword(s):

Embryo Development ◽

Bos Indicus ◽

Embryo Production ◽

University Of Illinois ◽

Oocyte Collection ◽

Sexed Semen ◽

The Usa ◽

In Vitro Embryo Production ◽

The University

The objective of this work was to compare in vitro embryo production of Bos taurus × Bos indicus cross embryos using oocytes from Holstein donors under different production and environment systems. This study also examined the possibility for in vitro production using oocytes imported and transported fresh between the USA and Panama. All animals were mature Holstein cows going through a normal lactation. The first group of donors was from the University of Illinois dairy herd and went through 3 ovum pickup sessions. The second group of donors were Holstein cows already adapted to Panama and went through 10 ovum pickup sessions. The Panamanian herd of Holstein donors were born and raised in Panama in an area of mountains, on average 1300 m above sea level. This environment does not have the typical hot and humid tropical weather seen in other regions of Panama. Both groups of donors were aspirated without stimulation during the years 2013 and 2014. Oocytes recovered from donors in Illinois were imported fresh under a special sanitary research protocol between Panama and the University of Illinois. The transport of fresh oocytes from the USA to Panama was done using a portable incubator set at 39°C (Minutube of America). Oocytes were matured during transport in 5-mL tubes (~30–35 oocytes per tube) containing 400 µL of maturation media (TCM-199) that had been equilibrated with 5% CO2. Oocytes recovered from donors in Panama were matured using the same media. For both groups, oocytes were inseminated 24 h after ovum pickup using sexed semen from the same bull. All embryo production procedures followed the protocols of the In vitro Brasil™ commercial system. At 72 h postinsemination, cleavage was evaluated. On Day 7 after insemination, embryo development to the blastocyst stage (early to expanded) was recorded. Data were analysed using Chi-squared. As shown in Table 1, there was no effect of oocyte collection location on embryo development. These results indicate that it is possible to produce a viable in vitro-produced embryo using fresh oocytes collected and transported from different countries. This work opens the possibility to access superior genetics and improve herds in countries seeking to increase their production systems and potentially improve their quality of life. Table 1.Effect of oocyte collection location on embryo development This project was supported by Programa de Competitividad ProCom Senacyt, Panama.

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In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH

Animal Reproduction Science ◽

10.1016/s0378-4320(00)00186-x ◽

2000 ◽

Vol 63 (3-4) ◽

pp. 145-158 ◽

Cited By ~ 30

Author(s):

K.L Goodhand ◽

M.E Staines ◽

J.S.M Hutchinson ◽

P.J Broadbent

Keyword(s):

Bovine Oocyte ◽

Embryo Production ◽

Oocyte Recovery ◽

In Vitro Embryo Production ◽

Oocyte Donors

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Transvaginal ultrasound-guided oocyte aspiration for production of embryos <i>in vitro</i>

Archives Animal Breeding ◽

10.5194/aab-45-99-2002 ◽

2002 ◽

Vol 45 (1) ◽

pp. 99-108

Author(s):

J. A. Carter ◽

S. Bellow ◽

M. Meintjes ◽

O. Perez ◽

E. Ferguson ◽

...

Keyword(s):

Transvaginal Ultrasound ◽

Farm Animals ◽

Embryo Research ◽

Ultrasound Guided ◽

Cyclic Activity ◽

Embryo Production ◽

Early Postpartum ◽

In Vitro Embryo Production ◽

Oocyte Aspiration

Abstract. reproductive potential in genetically valuable animals (BEAL et al., 1992). Now that repeatable oocyte retrieval methods are being fine-tuned, it is likely these procedures will become routinely used to obtain oocytes for further gamete and embryo research and also by seedstock producers for in vitro embryo production from farm animals in the commercial sector. The use of transvaginal ultrasound-guided oocyte aspiration and IVF procedure does offer an alternative to cattle producers who have genetically valuable cows that for some reason are unable to produce viable embryos through standard embryo collection procedures. This technology can be used on oocytes harvested from older ovulating or nonovulating cows, females with physical injuries (e.g., fractured leg) and problem cows having an abnormal cervix. Good success has been reported using IVF procedures on oocytes obtained from supplemental follicles of cows with cystic ovarian disease. With IVF the potential exists for more embryos to be produced in a shorter period of time, since the procedure can be repeated on the same cow 3 to 4 times or more a month. At this station, we are harvesting oocytes from early postpartum (< 40 days) beef and dairy cattle, before the female begins cyclic activity. The approach allows the opportunity to produce one or more extra calves from the cow before she is mated for a natural pregnancy. Currently, transvaginal ultrasound-guided oocyte aspiration is now being used to harvest valuable oocytes from minor farm animal breeds, from domestic females representing rare bloodlines, clinically infertile females and reproductively senescent cows. Research continues to find applications for this technology, including harvesting oocytes from young prepubertal heifers and early postpartum beef cows for in vitro embryo production. The use of ultrasound-guided oocyte aspiration should not be overlooked to obtain oocytes for in vitro embryo production and to aid in germplasm preservation of endangered exotic species.

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Transvaginal ultrasound-guided cumulus oocyte complexes aspiration and in vitro embryo production in suckled beef and lactating dairy cattle on pasture-based management conditions

Animal Reproduction Science ◽

10.1016/j.anireprosci.2011.10.001 ◽

2011 ◽

Vol 129 (1-2) ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

M.H. Ratto ◽

O.A. Peralta ◽

G. Mogollon ◽

P. Strobel ◽

J. Correa

Keyword(s):

Dairy Cattle ◽

Transvaginal Ultrasound ◽

Ultrasound Guided ◽

Embryo Production ◽

In Vitro Embryo Production ◽

Lactating Dairy Cattle

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In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200592680 ◽

1996 ◽

Vol 1996 ◽

pp. 68-68

Author(s):

K.L. Goodhand ◽

R.G. Watt ◽

M.E. Staines ◽

L.C. Higgins ◽

P.J. Broadbent ◽

...

Keyword(s):

Transvaginal Ultrasound ◽

Genetic Change ◽

Bovine Oocyte ◽

Ultrasound Guided ◽

Embryo Production ◽

Follicular Aspiration ◽

Oocyte Donors ◽

Pre Treatment

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

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Pregnancy obtained in a late gestational mare by in vitro embryo production

Reproduction Fertility and Development ◽

10.1071/rd19305 ◽

2019 ◽

Vol 31 (12) ◽

pp. 1926

Author(s):

Lino Fernando Campos-Chillon ◽

Jan Martin ◽

Joy L. Altermatt

Keyword(s):

Transvaginal Ultrasound ◽

Experimental Studies ◽

Late Gestation ◽

Follicular Growth ◽

Ultrasound Guided ◽

Embryo Production ◽

In Vitro Embryo Production

Recently, the demand for invitro embryo production in the horse has increased worldwide. Most clinical transvaginal ultrasound-guided ovum pick-up (OPU) procedures are performed in non-pregnant donor mares, and few experimental studies have described invitro embryo production from oocytes of pregnant donors 21–150 days in gestation. This report discusses OPU, follicular growth and invitro embryo production in a pregnant mare during late gestation.

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