'Real time' measurement of dopamine release in an in vitro model of neostriatal ischaemia

1996 ◽  
Vol 67 (2) ◽  
pp. 133-140 ◽  
Author(s):  
C Toner
Keyword(s):  
Real Time ◽  
Dopamine Release ◽  
In Vitro Model ◽  
Time Measurement ◽  
Real Time Measurement ◽  
Vitro Model

scholarly journals Real-time measurement of adenosine and ATP release in the central nervous system

2020 ◽  
Author(s):  
Nicholas Dale
Keyword(s):  
Real Time ◽  
Motor Pattern ◽  
Atp Release ◽  
In Vitro Models ◽  
Time Measurement ◽  
Pattern Generation ◽  
Neural Function ◽  
Adaptive Changes ◽  
Real Time Measurement

AbstractThis brief review recounts how, stimulated by the work of Geoff Burnstock, I developed biosensors that allowed direct real-time measurement of ATP and adenosine during neural function. The initial impetus to create an adenosine biosensor came from trying to understand how ATP and adenosine-modulated motor pattern generation in the frog embryo spinal cord. Early biosensor measurements demonstrated slow accumulation of adenosine during motor activity. Subsequent application of these biosensors characterized real-time release of adenosine in in vitro models of brain ischaemia, and this line of work has recently led to clinical measurements of whole blood purine levels in patients undergoing carotid artery surgery or stroke. In parallel, the wish to understand the role of ATP signalling in the chemosensory regulation of breathing stimulated the development of ATP biosensors. This revealed that release of ATP from the chemosensory areas of the medulla oblongata preceded adaptive changes in breathing, triggered adaptive changes in breathing via activation of P2 receptors, and ultimately led to the discovery of connexin26 as a channel that mediates CO2-gated release of ATP from cells.

scholarly journals A New 3D Cultured Liver Chip and Real-Time Monitoring System Based on Microfluidic Technology

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1118
Author(s):  
Yao Zhang ◽  
Ning Yang ◽  
Liangliang Xie ◽  
Fangyu Shu ◽  
Qian Shi ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Real Time ◽  
Monitoring System ◽  
Near Infrared Spectroscopy ◽  
In Vitro Model ◽  
Near Infrared ◽  
Real Time Monitoring ◽  
Microfluidic Technology ◽  
Vitro Model

In vitro models of the liver have a good simulation of the micro-liquid environment inside the human liver and the communication between cell tissues, which provides an important research tool for drug research and liver disease treatment. In this paper, we designed a 3D liver chip and real-time monitoring system based on microfluidic technology. The in vitro model of the liver on the chip was established by the three-dimensional microfluidic chip pipeline and the corresponding microwell array. Meanwhile, the culture medium is continuously injected on the chip, and the electrochemical impedance spectroscopy and near-infrared spectroscopy of the liver chip are recorded and analyzed from day one to day five. When the 3D cultured liver chip in vitro model reached a certain period and stabilized, paracetamol with varying gradients of concentration was applied to the cultured cells for drug resistance testing. The experimental results show that the liver chip and its monitoring system designed in this paper can maintain 100% cell viability of hepatocytes in vitro for a long time. Furthermore, it can meet the requirements of measurement technologies such as electrical impedance measurement and near-infrared spectroscopy in real-time, providing a stable culture platform for the further study of organ chips.

An in vitro model for studying acetylcholine and dopamine release from striatal slices

1989 ◽  
Vol 21 ◽  
pp. 75-76 ◽  
Author(s):  
L. Raimondi ◽  
S. Bianchi ◽  
C. Perego ◽  
S. Algeri
Keyword(s):  
Dopamine Release ◽  
In Vitro Model ◽  
Striatal Slices ◽  
Vitro Model
Sign in / Sign up

Export Citation Format

Share Document