Sequencing and heterologous expression of a cytosolic-type malate dehydrogenase of Trypanosoma brucei

2001 ◽  
Vol 117 (2) ◽  
pp. 217-221 ◽  
Author(s):  
Javier Vernal ◽  
Jorge Muñoz-Jordán ◽  
Miklós Müller ◽  
Juan José Cazzulo ◽  
Cristina Nowicki
Keyword(s):  
Heterologous Expression ◽  
Trypanosoma Brucei ◽  
Malate Dehydrogenase

Cloning, Heterologous Expression and Kinetic Analysis of Glycerol Kinase (TbGLK1) from Trypanosoma brucei

2000 ◽  
Vol 381 (11) ◽  
pp. 1071-1077 ◽  
Author(s):  
K. Steinborn ◽  
A. Szallies ◽  
D. Mecke ◽  
M. Duszenko
Keyword(s):  
Heterologous Expression ◽  
Kinetic Analysis ◽  
Trypanosoma Brucei ◽  
Blot Analysis ◽  
Spatial Separation ◽  
Single Copy ◽  
Glycerol Kinase ◽  
Mrna Levels ◽  
Atp Production ◽  
C Terminus

Abstract We have cloned and sequenced the gene for the glycerol kinase of Trypanosoma brucei (TbGLK1), obtained by RT-PCR. The corresponding mRNA is 2.3 kb in size and contains an ORF encoding a protein with high homology to known glycerol kinases of other organisms. It is 512 amino acids in length with a PTS1-like targeting sequence (AKL) at its C-terminus, suggesting glycosomal compartmentalization of this enzyme. Although Northern blot analysis revealed higher mRNA levels in slender bloodstream forms than in the procyclic insect forms, specific glycerol kinase activities were found to be virtually identical in both life stages. Southern blot analysis suggested a single copy gene, but we were able to clone two alleles utmost similar to each other. Heterologous expression of the trypanosomal glycerol kinase in E. coli enabled us to perform a kinetic analysis of this enzyme. In particular, we have been able to monitor ATP production from glycerol-3-phosphate and ADP, a reaction which, although thermodynamically very unfavorable, is regarded essential for the survival of Trypanosoma brucei under anoxic conditions. Since the unique spatial separation of glycolysis in the kinetoplastida imposes important consequences for the regulation of the energy metabolism in these organisms, we discuss the observed differences between TbGLK1 and glycerol kinases from other organisms in view of its physiological relevance.

scholarly journals Heterologous expression reveals distinct enzymatic activities of two DOT1 histone methyltransferases of Trypanosoma brucei

2010 ◽  
Vol 123 (23) ◽  
pp. 4019-4023 ◽  
Author(s):  
Floor Frederiks ◽  
Tibor van Welsem ◽  
Gideon Oudgenoeg ◽  
Albert J. R. Heck ◽  
Christian J. Janzen ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Trypanosoma Brucei ◽  
Enzymatic Activities ◽  
Histone Methyltransferases

Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage

Gene ◽  
2008 ◽  
Vol 411 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
Alice Machado-Silva ◽  
Santuza M.R. Teixeira ◽  
Glória R. Franco ◽  
Andréa M. Macedo ◽  
Sérgio D.J. Pena ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Oxidative Damage ◽  
Heterologous Expression ◽  
Mismatch Repair ◽  
Trypanosoma Brucei

Cloning and heterologous expression of the Trypanosoma brucei lanosterol synthase gene

2000 ◽  
Vol 110 (2) ◽  
pp. 399-403 ◽  
Author(s):  
Frederick S. Buckner ◽  
Lisa N. Nguyen ◽  
Bridget M. Joubert ◽  
Seiichi P.T. Matsuda
Keyword(s):  
Heterologous Expression ◽  
Trypanosoma Brucei ◽  
Lanosterol Synthase

The malate dehydrogenase isoforms from Trypanosoma brucei: Subcellular localization and differential expression in bloodstream and procyclic forms

2006 ◽  
Vol 36 (3) ◽  
pp. 295-307 ◽  
Author(s):  
Alejandro Aranda ◽  
Dante Maugeri ◽  
Antonio D. Uttaro ◽  
Fred Opperdoes ◽  
Juan. J. Cazzulo ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Differential Expression ◽  
Trypanosoma Brucei ◽  
Malate Dehydrogenase

Light-activation of NADP-malate dehydrogenase: A highly controlled process for an optimized function

2000 ◽  
Vol 110 (3) ◽  
pp. 322-329 ◽  
Author(s):  
M. Miginiac-Maslow ◽  
K. Johansson ◽  
E. Ruelland ◽  
E. Issakidis-Bourguet ◽  
I. Schepens ◽  
...  
Keyword(s):  
Malate Dehydrogenase ◽  
Light Activation
Sign in / Sign up

Export Citation Format

Share Document