Molecular cloning and structural characterization of the rat thymosin β15 gene

Gene ◽  
2000 ◽  
Vol 260 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Lere Bao ◽  
Bruce R. Zetter
Keyword(s):  
Molecular Cloning ◽  
Structural Characterization

Molecular cloning and structural characterization of Ecdysis Triggering Hormone from Choristoneura fumiferana

2016 ◽  
Vol 88 ◽  
pp. 213-221 ◽  
Author(s):  
Bhagath Kumar P. ◽  
Kasi Viswanath K. ◽  
Tuleshwori Devi S. ◽  
Sampath Kumar R. ◽  
Daniel Doucet ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Structural Characterization ◽  
Choristoneura Fumiferana ◽  
Ecdysis Triggering Hormone

Molecular Cloning and Structural Characterization of the Human Histidase Gene (HAL)

Genomics ◽  
1995 ◽  
Vol 29 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Mariko Suchi ◽  
Hirofumi Sano ◽  
Haruo Mizuno ◽  
Yoshiro Wada
Keyword(s):  
Molecular Cloning ◽  
Structural Characterization

scholarly journals Molecular Cloning and Structural Characterization of the Arg-gingipain Proteinase ofPorphyromonas gingivalis

1995 ◽  
Vol 270 (3) ◽  
pp. 1007-1010 ◽  
Author(s):  
Nadine Pavloff ◽  
Jan Potempa ◽  
Robert N. Pike ◽  
Vaclav Prochazka ◽  
Michael C. Kiefer ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Structural Characterization

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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