Detection of canine oral papillomavirus-DNA in canine oral squamous cell carcinomas and p53 overexpressing skin papillomas of the dog using the polymerase chain reaction and non-radioactive in situ hybridization

1998 ◽  
Vol 60 (2-4) ◽  
pp. 119-130 ◽  
Author(s):  
J Teifke
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Oral Squamous Cell Carcinomas ◽  
Polymerase Chain ◽  
Skin Papillomas ◽  
Papillomavirus Dna

scholarly journals A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus–2 Infection

2020 ◽  
Vol 57 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Elizabeth Alloway ◽  
Keith Linder ◽  
Susan May ◽  
Trevor Rose ◽  
Josepha DeLay ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Squamous Cell Carcinoma ◽  
Squamous Cell ◽  
Distant Metastases ◽  
Equus Caballus ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Potential Association ◽  
Polymerase Chain

Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As Equus caballus papillomavirus–2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.

Detection of human papillomavirus DNA by in situ hybridization and polymerase chain reaction in human papillomavirus equivocal and dysplastic cervical biopsies

1993 ◽  
Vol 24 (9) ◽  
pp. 1012-1016 ◽  
Author(s):  
Kenneth R. Shroyer ◽  
Glen S. Lovelace ◽  
Monica L. Abarca ◽  
Robert H. Fennell ◽  
Mary E. Corkill ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Papillomavirus Dna
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