Serial lectin affinity chromatography with concanavalin A and wheat germ agglutinin demonstrates altered asparagine-linked sugar-chain structures of prostatic acid phosphatase in human prostate carcinoma

1997 ◽  
Vol 695 (2) ◽  
pp. 439-443 ◽  
Author(s):  
Ken-ichiro Yoshida ◽  
Mikihiko Honda ◽  
Kyoko Arai ◽  
Yoshakatsu Hosoya ◽  
Hideo Moriguchi ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Concanavalin A ◽  
Prostate Carcinoma ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Prostatic Acid Phosphatase ◽  
Lectin Affinity Chromatography ◽  
Sugar Chain ◽  
Lectin Affinity ◽  
Human Prostate Carcinoma

scholarly journals Tyrosyl kinase activity is inversely related to prostatic acid phosphatase activity in two human prostate carcinoma cell lines

1986 ◽  
Vol 6 (12) ◽  
pp. 4753-4757
Author(s):  
M F Lin ◽  
C L Lee ◽  
G M Clinton
Keyword(s):  
Acid Phosphatase ◽  
Prostate Carcinoma ◽  
Kinase Activity ◽  
Carcinoma Cell ◽  
Prostatic Acid Phosphatase ◽  
Carcinoma Cells ◽  
Carcinoma Cell Lines ◽  
Endogenous Level ◽  
Human Prostate Carcinoma ◽  
Prostate Carcinoma Cell

Alterations in prostatic acid phosphatase (PAcP), a phosphotyrosyl phosphatase, corresponded to changes in overall tyrosyl kinase activity. PAcP added to extracts of prostate carcinoma cells with a low endogenous level of PAcP activity and elevated tyrosyl kinase activity decreased the tyrosyl kinase activity. On the other hand, when PAcP activity was decreased by the addition of androgens to cells, there was a corresponding increase in tyrosyl kinase activity.

The cellular level of prostatic acid phosphatase and the growth of human prostate carcinoma cells

1994 ◽  
Vol 57 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Ming-Fong Lin ◽  
Renee Garcia-Arenas ◽  
Xing-Zhong Xia ◽  
Barbara Biela ◽  
Fen-Fen Lin
Keyword(s):  
Acid Phosphatase ◽  
Prostate Carcinoma ◽  
Prostatic Acid Phosphatase ◽  
Cellular Level ◽  
Carcinoma Cells ◽  
Human Prostate ◽  
Human Prostate Carcinoma

The expression of prostatic acid phosphatase is transcriptionally regulated in human prostate carcinoma cells

1995 ◽  
Vol 111 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Renee Garcia-Arenas ◽  
Fen-Fen Lin ◽  
Dinlii Lin ◽  
Li-Ping Jin ◽  
Charles C.-Y. Shih ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Prostate Carcinoma ◽  
Prostatic Acid Phosphatase ◽  
Carcinoma Cells ◽  
Human Prostate ◽  
Human Prostate Carcinoma

scholarly journals Tyrosyl kinase activity is inversely related to prostatic acid phosphatase activity in two human prostate carcinoma cell lines.

1986 ◽  
Vol 6 (12) ◽  
pp. 4753-4757 ◽  
Author(s):  
M F Lin ◽  
C L Lee ◽  
G M Clinton
Keyword(s):  
Acid Phosphatase ◽  
Prostate Carcinoma ◽  
Kinase Activity ◽  
Carcinoma Cell ◽  
Prostatic Acid Phosphatase ◽  
Carcinoma Cells ◽  
Carcinoma Cell Lines ◽  
Endogenous Level ◽  
Human Prostate Carcinoma ◽  
Prostate Carcinoma Cell

Alterations in prostatic acid phosphatase (PAcP), a phosphotyrosyl phosphatase, corresponded to changes in overall tyrosyl kinase activity. PAcP added to extracts of prostate carcinoma cells with a low endogenous level of PAcP activity and elevated tyrosyl kinase activity decreased the tyrosyl kinase activity. On the other hand, when PAcP activity was decreased by the addition of androgens to cells, there was a corresponding increase in tyrosyl kinase activity.

scholarly journals Receptor-mediated gonadotropin action in the ovary. Inhibitory actions of concanavalin A and wheat-germ agglutinin on gonadotropin-stimulated cyclic AMP and progesterone responses in ovarian cells

1981 ◽  
Vol 200 (1) ◽  
pp. 153-159 ◽  
Author(s):  
S Azhar ◽  
K M Menon
Keyword(s):  
Cyclic Amp ◽  
Concanavalin A ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Carbohydrate Chain ◽  
Inhibitory Effect ◽  
Dolichos Biflorus ◽  
Acetylneuraminic Acid ◽  
Ovarian Cells ◽  
Proline Incorporation

Pretreatment of ovarian cells with concanavalin A and wheat-germ agglutinin blocked the gonadotropin-induced cyclic AMP and progesterone responses and this effect was time- and concentration-dependent. Basal production of either cyclic AMP or progesterone, however, was not affected by treatment of cells with lectin. The effect of concanavalin A on gonadotropin-mediated cyclic AMP and progesterone responses was blocked by alpha-methyl D-mannoside and alpha-methyl d-glucoside. Similarly the inhibitory effect of wheat-germ agglutinin was reversed by N-acetyl-D-glucosamine. Pretreatment of ovarian cells with concanavalin A or wheat-germ agglutinin had no effect on protein synthesis in the ovary as monitored by [3H]proline incorporation studies. Concanavalin A and wheat-germ agglutinin did not affect steroid production in response to dibutyryl cyclic AMP and 8-bromo cyclic AMP, indicating that the inhibitory action of lectin was occurring at a step before cyclic AMP formation. Lectins specific for L-fucose, D-galactose and N-acetyl-D-galactosamine, gorse seed agglutinin, peanut agglutinin and Dolichos biflorus agglutinin respectively, did not interfere with gonadotropin-induced cyclic AMP and progesterone responses. The present studies suggest that gonadotropin receptors may be glycoprotein in nature or closely associated with glycoprotein structures with the carbohydrate chain containing N-acetyl-D-glucosamine, mannose and possibly N-acetylneuraminic acid.

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