Acetaminophen–glutathione conjugate formation in a coupled cytochrome P-450-glutathione S-transferase assay system mediated by subcellular preparations from adult and weanling rat tissues

2002 ◽  
Vol 16 (6) ◽  
pp. 637-641 ◽  
Author(s):  
A Allameh ◽  
N Alikhani
Keyword(s):  
Assay System ◽  
Rat Tissues ◽  
Glutathione S Transferase ◽  
Glutathione Conjugate ◽  
Cytochrome P 450

Metabolism of n-hexane by rat liver and extrahepatic tissues and the effect of cytochrome P-450 inducers

1997 ◽  
Vol 16 (3) ◽  
pp. 131-137 ◽  
Author(s):  
Sarah J Crosbie ◽  
PG Blain ◽  
Faith M Williams
Keyword(s):  
Skeletal Muscle ◽  
Rat Liver ◽  
Fold Increase ◽  
Rat Tissues ◽  
Human Cytochrome ◽  
Extrahepatic Tissues ◽  
Mass Spectometry ◽  
Fast Twitch ◽  
Cytochrome P 450

1 The in vitro metabolism ofn-hexane was studied in rat liver, lung, brain and skeletal muscle microsomes and in microsomes prepared from cell lines expressing human cytochrome P-450 2E1 or 2B6. The hydro xylated metabolites ofn-hexane were quantified by gas chromatography-mass spectometry. 2 Rat liver and extensor digitorum longus (EDL, fast- twitch skeletal muscle) microsomes and the CYP 2B6 microsomes produced the pre-neurotoxic metabolite of n-hexane, 2-hexanol as a major metabolite in contrast to the other rat tissues examined. 3 Inhibition of 2- and 3-hexanol production from n- hexane by rat lung microsomes using metyrapone, an inhibitor of cytochrome P-450 2B1 activity, resulted in almost complete inhibition of lung microsomal activ ity. 4 Production of all three hexanols was significantly increased with phenobarbital-induced rat liver micro somes, with a 10-fold increase in 2- and 3-hexanol production. A slight increase in 2-hexanol production with phenobarbital-induced rat EDL and brain micro somes was observed. No increase in n-hexane meta bolism was noted following induction with β- naphthoflavone or with ethanol.

Interactions of glutathione S-transferase-π with ethacrynic acid and its glutathione conjugate

1993 ◽  
Vol 1164 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Sanjay Awasthi ◽  
Sanjay K. Srivastava ◽  
Faiyaz Ahmad ◽  
Hassan Ahmad ◽  
G.A.S. Ansari
Keyword(s):  
Ethacrynic Acid ◽  
Glutathione S Transferase ◽  
Glutathione Conjugate
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