Automated data collection for simulation?

2002 ◽  
Vol 9 (6-8) ◽  
pp. 349-364 ◽  
Author(s):  
N Robertson ◽  
T Perera
Author(s):  
Weiping Liu ◽  
Jennifer Fung ◽  
W.J. de Ruijter ◽  
Hans Chen ◽  
John W. Sedat ◽  
...  

Electron tomography is a technique where many projections of an object are collected from the transmission electron microscope (TEM), and are then used to reconstruct the object in its entirety, allowing internal structure to be viewed. As vital as is the 3-D structural information and with no other 3-D imaging technique to compete in its resolution range, electron tomography of amorphous structures has been exercised only sporadically over the last ten years. Its general lack of popularity can be attributed to the tediousness of the entire process starting from the data collection, image processing for reconstruction, and extending to the 3-D image analysis. We have been investing effort to automate all aspects of electron tomography. Our systems of data collection and tomographic image processing will be briefly described.To date, we have developed a second generation automated data collection system based on an SGI workstation (Fig. 1) (The previous version used a micro VAX). The computer takes full control of the microscope operations with its graphical menu driven environment. This is made possible by the direct digital recording of images using the CCD camera.


1983 ◽  
Author(s):  
Peter J. Kennedy ◽  
James R. Lewis

2003 ◽  
Author(s):  
Daniel K. Fisher ◽  
Steven J. Thomson ◽  
Lowrey A. Smith ◽  
Brian L. Brazil

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