Characterization of 11-dehydro-thromboxane B2 recombinant antibody obtained by phage display technology

2003 ◽  
Vol 68 (4) ◽  
pp. 273-284 ◽  
Author(s):  
Lilian Rumi Tsuruta ◽  
Yoshihisa Tomioka ◽  
Takanori Hishinuma ◽  
Yoshinori Kato ◽  
Kunihiko Itoh ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Thromboxane B2 ◽  
Phage Display Technology ◽  
Display Technology

scholarly journals Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7652
Author(s):  
Wei Sun ◽  
Yan Zhang ◽  
Zhigang Ju
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Recombinant Antibodies ◽  
Phage Display Library ◽  
Ease Of Use ◽  
Phage Library ◽  
Phage Display Technology ◽  
Random Peptide ◽  
Display Technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

Production and Characterization of Recombinant scFv Against Digoxin by Phage Display Technology

2013 ◽  
Vol 32 (3) ◽  
pp. 172-179 ◽  
Author(s):  
Behruz Alirezapour ◽  
Masoumeh Rajabibazl ◽  
Mohhamad Javad Rasaee ◽  
Kobra Omidfar
Keyword(s):  
Phage Display ◽  
Phage Display Technology ◽  
Recombinant Scfv ◽  
Display Technology

Targeting Cytokines: Production and Characterization of Anti-TNF-α scFvs by Phage Display Technology

2013 ◽  
Vol 19 (15) ◽  
pp. 2839-2847 ◽  
Author(s):  
Jalal Abdolalizadeh ◽  
Mohammad Nouri ◽  
Jafar Majidi Zolbanin ◽  
Abolfazl Barzegari ◽  
Behzad Baradaran ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Technology ◽  
Tnf Α ◽  
Display Technology

Characterization of prostate-specific antigen binding peptides selected by phage display technology

2005 ◽  
Vol 19 (1) ◽  
pp. 10-20 ◽  
Author(s):  
Catherine Ferrieu-Weisbuch ◽  
Sandrine Michel ◽  
Emilie Collomb-Clerc ◽  
Catherine Pothion ◽  
Gilbert Deléage ◽  
...  
Keyword(s):  
Phage Display ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Binding ◽  
Phage Display Technology ◽  
Display Technology ◽  
Binding Peptides

scholarly journals Characterization of Novel Monoclonal Antibodies for Prostate-Specific Antigen (PSA) with Potency to Recognize PSA Bound to α2-Macroglobulin

2005 ◽  
Vol 51 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Yvonne Baumgart ◽  
Andreas Otto ◽  
Angelika Schäfer ◽  
Elke Usbeck ◽  
Christiane Cott ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Molecular Forms ◽  
Phage Display Technology ◽  
Free Psa ◽  
Α2 Macroglobulin ◽  
Display Technology

Abstract Background: Different molecular forms of prostate-specific antigen (PSA) have been used to differentiate between benign prostatic hyperplasia and prostate cancer. Detecting PSA bound to endogenous inhibitors such as α1-antichymotrypsin (ACT) and α2-macroglobulin (α2M) is often difficult because of epitope masking or sensitivity problems. Here we report the characterization of four novel mouse monoclonal antibodies (mabs) obtained by immunization with PSA-α2M complexes. Their ability to detect free PSA and PSA-inhibitor complexes was shown, and their epitopes were analyzed by phage display technology. Methods: The properties of the mabs were studied by competition and sandwich assays and by Western blotting. Epitope mapping was performed by screening of a phage display peptide library. Results: All four mabs recognized free PSA, PSA-ACT, and PSA-α2M complexes, but to various degrees. With different combinations of mabs in competition experiments, antibodies were identified that enhance binding of other mabs to PSA, forming the molecular basis of a very sensitive assay for the detection of PSA and PSA-ACT complexes. Mabs with highest reactivity for PSA-α2M were selected to establish an immunoassay for that complex. Western blot analysis revealed that all mabs recognized conformational epitopes of PSA. These findings were supported by phage display results demonstrating mimotopes in the PSA molecule. Conclusion: The results presented here could aid in the further development of clinically relevant assays for PSA and PSA-α2M complexes.

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