Cytochemical localization of adenylate cyclase activity in the symbiotic protocorms of Spiranthes sinensis

1996 ◽  
Vol 100 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Yukari Uetake ◽  
Nobuyuki Ishizaka
1979 ◽  
Vol 35 (10) ◽  
pp. 1315-1317 ◽  
Author(s):  
Rita Pascolini ◽  
Anna Maria Gargiulo ◽  
A. Spreca ◽  
A. Orlacchio

1982 ◽  
Vol 223 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Rita Pascolini ◽  
A. M. Gargiulo ◽  
A. Spreca ◽  
P. Ceccarelli

Development ◽  
1976 ◽  
Vol 36 (2) ◽  
pp. 291-303
Author(s):  
Leslie S. Cutler ◽  
Sevgi B. Rodan

To investigate membrane changes in development of the exocrine cells of the rat submandibular gland (SMG), biochemical and cytochemical studies of adenylate cyclase activity were performed on prenatal and postnatal glands. SMG rudiments and glands were studied from 15 days of gestation up to birth and 1, 2, 3, 4 and 24 weeks after birth. Glands were chemically assayed for adenylate cyclase activity using the procedures of Salomon and coworkers and cytochemically studied using a procedure which was verified biochemically. At 15–16 days of gestation basal adenylate cyclase activity was low and no staining could be observed. Adenylate cyclase activity rose six-fold from the 16th to the 18th day of gestation. Adenylate cyclase staining became evident along the surface of most of the cells of the rudiment at this time. Basal adenylate cyclase activity remained relatively constant from the 18th day of gestation up to 24 weeks of age. However, sequential changes were seen in the cytochemical localization, especially in relation to the apical plasma membrane of the developing secretory cells.


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