scholarly journals The cytochemical localization of adenylate cyclase activity in mucous and serous cells of the salivary gland

1976 ◽  
Vol 4 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Sun-Kee Kim
Keyword(s):  
Salivary Gland ◽  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Localization

scholarly journals Regulation of adenylate cyclase and cyclic AMP phosphodiesterase by 5-hydroxytryptamine and calcium ions in blowfly salivary-gland homogenates

1982 ◽  
Vol 204 (1) ◽  
pp. 153-159 ◽  
Author(s):  
I Litosch ◽  
M Fradin ◽  
M Kasaian ◽  
H S Lee ◽  
J N Fain
Keyword(s):  
Salivary Gland ◽  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Guanine Nucleotides ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cyclic Amp Phosphodiesterase ◽  
Maximal Stimulation ◽  
Increased Activity ◽  
Stimulation Of

Salivary-gland homogenates contain 5-hydroxytryptamine-stimulated adenylate cyclase. Half-maximal stimulation was obtained with 0.1 microM-5-hydroxytryptamine in the presence of added guanine nucleotides. Gramine antagonized the stimulation of cyclase caused by 5-hydroxytryptamine. In the presence of hormone, guanosine 5′-[gamma-thio]triphosphate produced a marked activation of adenylate cyclase activity. Stimulation of adenylate cyclase by forskolin or fluoride did not require the addition of guanine nucleotides or hormone. In the presence of EGTA, Ca2+ produced a biphasic activation of cyclase activity. Ca2+ at 1-100 microM increased activity, whereas 2000 microM-Ca2+ inhibited cyclase activity. The neuroleptic drugs trifluoperazine and chlorpromazine non-specifically inhibited adenylate cyclase activity even in the absence of Ca2+. The cyclic AMP phosphodiesterase activity in homogenates was not affected by Ca2+ or exogenous calmodulin. This enzyme was also inhibited by trifluoperazine in the absence of Ca2+. These results indicate that Ca2+ elevates adenylate cyclase activity, but had no effect on cyclic AMP phosphodiesterase of salivary-gland homogenates.

Studies on the cytochemical localization of adenylate-cyclase activity inDugesia lugubris s.l.

1979 ◽  
Vol 35 (10) ◽  
pp. 1315-1317 ◽  
Author(s):  
Rita Pascolini ◽  
Anna Maria Gargiulo ◽  
A. Spreca ◽  
A. Orlacchio
Keyword(s):  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Localization

Cytochemical localization of adenylate cyclase activity in planarian tissues

1982 ◽  
Vol 223 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Rita Pascolini ◽  
A. M. Gargiulo ◽  
A. Spreca ◽  
P. Ceccarelli
Keyword(s):  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Cytochemical Localization
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