Characterization of subclones of the epithelial cell line from Chironomus tentans resistant to the insecticide RH 5992, a non-steroidal moulting hormone agonist

2000 ◽  
Vol 30 (7) ◽  
pp. 591-600 ◽  
Author(s):  
Marco Grebe ◽  
Peter Rauch ◽  
Margarethe Spindler-Barth
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Chironomus Tentans ◽  
Moulting Hormone

Hormonal Regulation of Acetylcholinesterase in an Epithelial Cell Line from Chironomus tentans

1991 ◽  
Vol 46 (11-12) ◽  
pp. 1089-1093 ◽  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Hormonal Regulation ◽  
Maximal Response ◽  
Epithelial Cell Line ◽  
Chironomus Tentans ◽  
Solubilized Enzyme ◽  
Membrane Bound ◽  
Globular Form ◽  
Moulting Hormone

Acetylcholinesterase (AchE) can be induced in a non-neuronal, epithelial cell line from Chironomus tentans by the moulting hormone 20-OH-ecdysone. Maximal response is reached after 7 days. The increase in enzymatic activity can be suppressed by simultaneous incubation with inhibitors of transcription and translation. The potency of various steroid hormones to induce AchE coincides with their affinity to the ecdysteroid hormone receptor. AchE from Chironomus is tightly bound to membranes and cannot be solubilized by heparin or collagenase treatment. Incubation under conditions where phospholipase C is most active releases activity into the supernatant. If phospholipase is applied in addition, the amount of solubilized enzyme increases, but there is still membrane-bound activity left. This is in accordance with a globular form of AchE linked to membranes by a phospholipid-anchor. The enzyme sediments as a single peak of 5.4 S.

Establishment and characterization of a SV40 T-antigen immortalized human bronchial epithelial cell line

1992 ◽  
Vol 28 (7-8) ◽  
pp. 461-464 ◽  
Author(s):  
Joan H. Schiller ◽  
Chinghai Kao ◽  
Gerard Bittner ◽  
Chuck Harris ◽  
Terry D. Oberley ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Bronchial Epithelial Cell ◽  
T Antigen ◽  
Human Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Sv40 T Antigen ◽  
Bronchial Epithelial

CHARACTERIZATION OF JOK-1, A HUMAN GASTRIC EPITHELIAL CELL LINE

2000 ◽  
Vol 36 (4) ◽  
pp. 228-234
Author(s):  
N. OKAYAMA ◽  
M. R. FOWLER ◽  
S. R. JENNINGS ◽  
P. PATEL ◽  
R. SPECIAN ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Gastric Epithelial Cell ◽  
Epithelial Cell Line
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