Perivenous application of fibrin glue reduces early injury to the human saphenous vein graft wall in an ex vivo model

Objective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-α and determination of mRNA level alterations of ICAM-1 and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. Results. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-α group was significantly higher than that in the TNF-α group and significantly lower than that in the high-dose pitavastatin+TNF-α group (for all comparisons, P = 0.001). The low-dose pitavastatin+TNF-α group had a similar NF-κB mRNA expression with TNF-α and high-dose pitavastatin+TNF-α groups. Conclusion. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field.

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Effects of Antioxidants on Endothelial Function in Human Saphenous Vein in an Ex vivo Model

Angiology ◽

10.1177/0003319708321186 ◽

2008 ◽

Vol 60 (4) ◽

pp. 448-454 ◽

Cited By ~ 3

Author(s):

Muhammed Anees Sharif ◽

Ulvi Bayraktutan ◽

Nityanand Arya ◽

Stephen A. Badger ◽

Mark E. O'Donnell ◽

...

Keyword(s):

Vitamin C ◽

Endothelial Function ◽

Saphenous Vein ◽

Ex Vivo ◽

High Dose ◽

Organ Bath ◽

Human Saphenous Vein ◽

Ex Vivo Model ◽

Antioxidant Agents ◽

Endothelium Dependent Relaxation

This ex vivo study is aimed at determining the beneficial effects of antioxidant agents on human saphenous vein endothelial function. Vein rings harvested during infrainguinal bypass surgery were assessed in an organ bath for endothelium-dependent relaxation, initially without and then with the addition of 10 μM manganese tetrakis benzoic acid porphyrin (MnTBAP), 0.01% N-acetylcysteine (NAC), 0.02% NAC, 10 μM vitamin C, and 100 μM vitamin C. Fifty-five vein rings from 22 patients were analyzed. MnTBAP improved the endothelium-dependent relaxation when compared with control (57.0% vs 37.8%, P < .01). Addition of 0.01% or 0.02% NAC did not improve the endothelium-dependent vasorelaxation (28.2% vs 18.6%, P = ns and 37.8% vs 29.8%, P = ns, respectively). Although 10-μM vitamin C failed to improve endothelial function (50.6% vs 37.2%, P = ns), 100-μM vitamin C significantly enhanced endothelium-dependent relaxation (66.5% vs 38.3%, P < .001). These results suggest that the addition of MnTBAP and high-dose vitamin C can improve the endothelial function of harvested saphenous vein segments in an ex vivo model.

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