Metastable decay of negatively charged oligodeoxynucleotides analyzed with ultraviolet matrix-assisted laser desorption/ionization post-source decay and deuterium exchange

2001 ◽  
Vol 12 (2) ◽  
pp. 180-192 ◽  
Author(s):  
Julia Gross ◽  
Franz Hillenkamp ◽  
Katty X. Wan ◽  
Michael L. Gross
Keyword(s):  
Laser Desorption ◽  
Deuterium Exchange ◽  
Laser Desorption Ionization ◽  
Post Source Decay ◽  
Metastable Decay ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Post-Source Decay Analysis of Dolastatin 10 and Dolastatin 15 by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2002 ◽  
Vol 8 (4) ◽  
pp. 311-321 ◽  
Author(s):  
Nathalie Galéotti ◽  
Joël Poncet ◽  
Patrick Jouin
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Post Source Decay ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption ◽  
Dolastatin 10

Dolastatin 10 and dolastatin 15 have been analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry. Interestingly, in-source fragmentations could be easily detected for both compounds. Determination of the primary structure of both pseudopeptides could be done by using post-source decay (PSD) analysis. The molecular ion was used as the precursor ion in the case of dolastatin 10, whereas two in-source fragment ions and cationized (Na and K) precursors were selected in the case of dolastatin 15. An analogue of dolastatin 15 containing an amino alkyl linkage (compound 1) was also studied. Despite its close chemical structure its PSD spectrum exhibited a quite different pattern.

Changes in Post-Source Decay Fragmentation Behavior of Poly(Methyl Methacrylate) Polymers with Increasing Molecular Weight Studied by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2003 ◽  
Vol 9 (3) ◽  
pp. 195-201 ◽  
Author(s):  
Olli Laine ◽  
Sarah Trimpin ◽  
Hans Joachim Räder ◽  
Klaus Müllen
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Post Source Decay ◽  
Ionization Time ◽  
Fragmentation Behavior ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

In order to investigate the systematic changes in fragmentation behavior of poly(methyl methacrylate) (PMMA) with increasing molecular weight, alkali–metal cationized PMMA 20-mer, 60-mer and 100-mer were selected for post-source decay (PSD) fragmentation study by matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry. PMMA polymers were cationized with lithium, potassium and cesium cations to explore the influence of the cation size on the fragmentation behavior of the polymers. All PMMA polymers could be fragmented by MALDI-PSD and fragmentation of the MALDI ionized synthetic polymer of molecular weight 10 kDa is reported here for the first time. It was shown that an increasing molecular weight of the PMMA chain required an increase in the size of the cation to improve the intensity and the number of the fragments in the PSD spectrum. Some instrumental parameters had to be optimized prior to a successful PSD analysis of the largest PMMA polymers.

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