Inability to Detect Cell Free Fetal DNA in the Urine of Normal Pregnant Women nor in Those Affected by Preeclampsia Associated HELLP Syndrome

Sixty blood samples from pregnant women during gestational weeks 9–28 were investigated. Cell-free fetal DNA was extracted from maternal plasma or serum to be detected by nested PCR for determination of fetal gender. The SRY gene as a marker for fetal Y chromosome was detected in 34/36 women carrying a male fetus. In 3/24 women carrying female fetuses, the SRY sequence was also detected. Overall, fetal sex was correctly predicted in 91.7% of the cases. Therefore, the new, non-invasive method of prenatal diagnosis of fetal gender for women at risk of producing children with X-linked disorders is reliable, secure, and can substantially reduce invasive prenatal tests.

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Identifying mild and severe preeclampsia in asymptomatic pregnant women by levels of cell-free fetal DNA

Transfusion ◽

10.1111/trf.12073 ◽

2013 ◽

Vol 53 (9) ◽

pp. 1956-1964 ◽

Cited By ~ 17

Author(s):

Tanja Roien Jakobsen ◽

Frederik Banch Clausen ◽

Line Rode ◽

Morten Hanefeld Dziegiel ◽

Ann Tabor

Keyword(s):

Pregnant Women ◽

Severe Preeclampsia ◽

Fetal Dna ◽

Cell Free Fetal Dna

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An Effective Method for Detecting Y-chromosome Specific Sequences of Circulating Fetal DNA in Maternal Plasma During the First-trimester

International Journal of Medical Laboratory ◽

10.18502/ijml.v6i2.1025 ◽

2019 ◽

Author(s):

Najmeh Davoodian ◽

Ali Kadivar ◽

Heidar Heidari Khoie ◽

Sima Hematian Khayat ◽

Mahboobeh Heidari Nasirabadi

Keyword(s):

Prenatal Diagnosis ◽

Pregnant Women ◽

Real Time ◽

Y Chromosome ◽

Maternal Plasma ◽

Fetal Sex ◽

Non Invasive ◽

Fetal Dna ◽

Fetal Gender ◽

Cell Free Fetal Dna

Background and Aims: New advances in the use of cell-free fetal DNA (cffDNA) in maternal plasma of pregnant women has provided the possibility of applying cffDNA in prenatal diagnosis as a non-invasive method. One of the applications of prenatal diagnosis is fetal gender determination. Early prenatal determination of fetal sex is required for pregnant women at risk of X-linked and some endocrine diseases. The present study was carried out to perform an efficient polymerase chain reaction (PCR) method in order to improve sensitivity, specificity and accuracy of non-invasive fetal gender detection using fetal DNA in maternal plasma during 8th -12th weeks of pregnancy. Materials and Methods: Thirty-five pregnant women with 8 to 12 weeks of pregnancy were selected for prenatal fetal sex determination. Maternal peripheral blood was collected and cffDNA was extracted from 3-ml of maternal plasma. Two multi copy Y-chromosome-specific region (DYS and DAZ) and a single copy gene (SRY) were amplified by real-time quantitative PCR. Amplification was labeled as positive, negative, or inconclusive according to a stringent algorithm. Results: Using this method, the sensitivity and specificity of the real-time PCR assay was 100% and 93.8% for prenatal fetal sex detection, respectively. Conclusions: It is concluded that fetal sex can be determined with a high level of accuracy by our algorithm, after 8 weeks of gestation with cffDNA analysis.

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