scholarly journals 161. Analysis of Target Site Selection for the Sleeping Beauty Transposon in Mouse Liver

2003 ◽  
Vol 7 (5) ◽  
pp. S63
Keyword(s):  
Site Selection ◽  
Mouse Liver ◽  
Sleeping Beauty ◽  
Target Site ◽  
Target Site Selection ◽  
Sleeping Beauty Transposon ◽  
Selection For

Mapping target site selection for the non-specific nuclease activities of retroviral integrase

2000 ◽  
Vol 66 (1) ◽  
pp. 87-100 ◽  
Author(s):  
Michael Katzman ◽  
Malgorzata Sudol ◽  
Jeffrey S. Pufnock ◽  
Shawn Zeto ◽  
Lynn M. Skinner
Keyword(s):  
Site Selection ◽  
Target Site ◽  
Target Site Selection ◽  
Selection For ◽  
Retroviral Integrase

Target site selection for an RNA-cleaving catalytic DNA

10.1038/8658 ◽  
1999 ◽  
Vol 17 (5) ◽  
pp. 480-486 ◽  
Author(s):  
Murray J. Cairns ◽  
Toni M. Hopkins ◽  
Craig Witherington ◽  
Li Wang ◽  
Lun-Quan Sun
Keyword(s):  
Site Selection ◽  
Target Site ◽  
Target Site Selection ◽  
Selection For ◽  
Catalytic Dna

Ezrin mRNA target site selection for DNAzymes using secondary structure and hybridization thermodynamics

Tumor Biology ◽  
2011 ◽  
Vol 32 (4) ◽  
pp. 809-817 ◽  
Author(s):  
YaoFei Wang ◽  
JingNan Shen ◽  
XiFu Shang ◽  
Jin Wang ◽  
JingChun Li ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Site Selection ◽  
Target Site ◽  
Target Site Selection ◽  
Selection For

Common Physical Properties of DNA Affecting Target Site Selection of Sleeping Beauty and other Tc1/mariner Transposable Elements

2002 ◽  
Vol 323 (3) ◽  
pp. 441-452 ◽  
Author(s):  
Thomas J. Vigdal ◽  
Christopher D. Kaufman ◽  
Zsuzsanna Izsvák ◽  
Daniel F. Voytas ◽  
Zoltán Ivics
Keyword(s):  
Transposable Elements ◽  
Physical Properties ◽  
Site Selection ◽  
Sleeping Beauty ◽  
Target Site ◽  
Target Site Selection ◽  
Selection Of

scholarly journals The integration preference of Sleeping Beauty at non-TA site is related to the transposon end sequences

2019 ◽  
Author(s):  
Yiting Zhou ◽  
Guangwei Ma ◽  
Jiawen Yang ◽  
Yabin Guo
Keyword(s):  
Site Selection ◽  
Genomic Dna ◽  
Consensus Sequence ◽  
Mouse Cell ◽  
Sleeping Beauty ◽  
Consensus Sequences ◽  
Target Site Selection ◽  
Target Sites ◽  
End Sequences ◽  
Selection Of

Abstract Background: Sleeping Beauty (SB) transposon had been thought to strictly integrate into TA dinucleotides. Recently, we found that SB also integrates into non-TA sites at a lower frequency. Here we performed further study on the non-TA integration of SB. Results: 1) SB can integrate into non-TA sites in HEK293T cells as well as in mouse cell lines. 2) Both the hyperactive transposase SB100X and the traditional SB11 catalyze integrations at non-TA sites. 3) The consensus sequence of the non-TA target sites only occur at the opposite side of the sequenced junction between transposon end and the genomic sequences, indicating that the integrations at non-TA sites are mainly aberrant integrations. 4) The consensus sequence of the non-TA target sites is corresponding to the transposon end sequence. When the transposon end sequence is mutated, the consensus sequences changed too. Conclusion: The interaction between the SB transposon end and genomic DNA may be involved in the target site selection of the SB integrations at non-TA sites.

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