Sebaceous Basal Cell Carcinoma - An Ultrastructural Study

Basal cell carcinoma with sebaceous gland differentiation has been described in mice and humans but spontaneous skin tumors of this type are uncommon in the rat.The tumor specimen was taken from a 26 month old male Wistar rat. Electron microscopy specimens were fixed by immersion in cacodylatebuffered 4% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated, cleared and embedded by standard procedures. Semi-thin (μm) sections were stained with ethanolic uranyl acetate followed by lead citrate, then examined on a Philips EM201 electron microscope.

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Ultrastructure of Mouse Basal Cell Carcinoma Exhibiting Sebaceous Differentiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003214 ◽

1980 ◽

Vol 38 ◽

pp. 738-739

Author(s):

Victoria L. Wade ◽

Winslow G. Sheldon ◽

James W. Townsend ◽

William Allaben

Keyword(s):

Basal Cell Carcinoma ◽

Cell Carcinoma ◽

Topical Application ◽

Basal Cell ◽

Sebaceous Gland ◽

Rats And Mice ◽

Mixed Tumors

Sebaceous gland tumors and other tumors exhibiting sebaceous differentiation have been described in humans (1,2,3). Tumors of the sebaceous gland can be induced in rats and mice following topical application of carcinogens (4), but spontaneous mixed tumors of basal cell origin rarely occur in mice.

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Recurrent sebaceous gland carcinoma of eyelid previously diagnosed as basal cell carcinoma: case report

American Journal of Otolaryngology ◽

10.1016/j.amjoto.2010.10.001 ◽

2011 ◽

Vol 32 (6) ◽

pp. 620-623 ◽

Cited By ~ 2

Author(s):

Li-Wei Kan ◽

Yi-Shing Leu ◽

Chi-Yuan Tzen ◽

Chien-Hsiu Wu

Keyword(s):

Case Report ◽

Basal Cell Carcinoma ◽

Cell Carcinoma ◽

Basal Cell ◽

Sebaceous Gland ◽

Sebaceous Gland Carcinoma ◽

Gland Carcinoma

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Membranous alterations in the cytoplasm and nucleus in a primitive neuroectodermal tumor of the brain

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084016 ◽

1978 ◽

Vol 36 (2) ◽

pp. 628-629

Author(s):

C. N. Sun ◽

C. Araoz ◽

H. J. White

Keyword(s):

Nuclear Envelope ◽

Tumor Cells ◽

Osmium Tetroxide ◽

Primitive Neuroectodermal Tumor ◽

Uranyl Acetate ◽

Neuroectodermal Tumor ◽

Annulate Lamellae ◽

Lead Citrate ◽

Thin Sections ◽

The Brain

The ultrastructure of a cerebral primitive neuroectodermal tumor has been reported previously. In the present case, we will present some unusual previously unreported membranous structures and alterations in the cytoplasm and nucleus of the tumor cells.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4% glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1% buffered osmium tetroxide for one hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate.In the cytoplasm of the tumor cells, we found paired cisternae (Fig. 1) and annulate lamellae (Fig. 2) noting that the annulate lamellae were sometimes associated with the outer nuclear envelope (Fig. 3). These membranous structures have been reported in other tumor cells. In our case, mitochondrial to nuclear envelope fusions were often noted (Fig. 4). Although this phenomenon was reported in an oncocytoma, their frequency in the present study is quite striking.

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Banded Structures in the Connective Tissue of Meningioma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100091159 ◽

1976 ◽

Vol 34 ◽

pp. 234-235

Author(s):

C. N. Sun ◽

H. J. White

Keyword(s):

Connective Tissue ◽

Osmium Tetroxide ◽

Uranyl Acetate ◽

Collagen Fibrils ◽

Lead Citrate ◽

Connective Tissues ◽

Native Collagen ◽

Routine Procedures

Previously, we have reported on extracellular cross-striated banded structures in human connective tissues of a variety of organs (1). Since then, more material has been examined and other techniques applied. Recently, we studied a fibrocytic meningioma of the falx. After the specimen was fixed in 4% buffered glutaraldehyde and post-fixed in 1% buffered osmium tetroxide, other routine procedures were followed for embedding in Epon 812. Sections were stained with uranyl acetate and lead citrate. There were numerous cross striated banded structures in aggregated bundle forms found in the connecfive tissue of the tumor. The banded material has a periodicity of about 450 Å and where it assumes a filamentous arrangement, appears to be about 800 Å in diameter. In comparison with the vicinal native collagen fibrils, the banded material Is sometimes about twice the diameter of native collagen.

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The Identification of Phospholipid Micelles in Glutaraldehyde-Urea Embedded Tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116097 ◽

1975 ◽

Vol 33 ◽

pp. 494-495

Author(s):

Daniel C. Pease

Keyword(s):

Electron Micrographs ◽

Lung Tissue ◽

Osmium Tetroxide ◽

Uranyl Acetate ◽

Type Ii ◽

Lead Citrate ◽

Phospholipid Micelles ◽

Type Ii Pneumocytes ◽

Ultrathin Sections ◽

Polar Water

It is reasonable to think that phospholipid micelles should be visible and identifiable in electron micrographs of ultrathin sections if only they can be preserved throughout the embedding process. The development of highly polar, water-containing, aminoplastic embedments has made this a likely possibility. With this in mind, an investigation of the lecithin-secreting, Type II pneumocytes of the lung is underway.Initially it has been easiest to recognize phospholipid micelles in lung tissue fixed first with glutaraldehyde, and then secondarily exposed to osmium tetroxide. However, the latter is not a necessary concomitant for micellar preservation. Conventional uranyl acetate and lead citrate staining is finally applied. Importantly, though, the micelles have been most easily seen in tissue embedded in 507. glutaraldehyde polymerized with urea, as described in detail by D.C. Pease and R.G. Peterson (J. Ultra- struct. Res., 41, 133, 1972). When oriented appropriately, the micellar units are seen as tiny, bilayer plates.

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