Phylogenetic relationships of the genus Phanerochaete inferred from the internal transcribed spacer region

Three dictyostelid isolates were found in Spain and Argentina that are morphologically different from known species. These isolates have some features similar to Dictyostelium sphaerocephalum (Oudem.) Sacc., Marchal & É.J. Marchal, but differ in size and sorocarp branching pattern. We sequenced the nuclear ribosomal internal transcribed spacer region to explore phylogenetic relationships among this group of species, including the three new isolates and their closest relatives. In all phylogenetic analyses performed, sequences of all three isolates group together with sequences from “typical” D. sphaerocephalum samples. This result supports previous observations of the morphological plasticity in dictyostelids, especially D. sphaerocephalum, leading us to broaden the classical concept of this species.

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Phylogenetic Relationships in Helianthus (Asteraceae) Based on Nuclear Ribosomal DNA Internal Transcribed Spacer Region Sequence Data

Systematic Botany ◽

10.2307/2419587 ◽

1998 ◽

Vol 23 (2) ◽

pp. 177 ◽

Cited By ~ 43

Author(s):

Edward E. Schilling ◽

C. Randal Linder ◽

Richard D. Noyes ◽

Loren H. Rieseberg

Keyword(s):

Ribosomal Dna ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Nuclear Ribosomal Dna ◽

Internal Transcribed Spacer Region ◽

Region Sequence

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Reconstruction of phylogenetic relationships in dermatomycete genusTrichophytonMalmsten 1848 based on ribosomal internal transcribed spacer region, partial 28S rRNA and beta-tubulin genes sequences

Mycoses ◽

10.1111/myc.12505 ◽

2016 ◽

Vol 59 (9) ◽

pp. 566-575 ◽

Cited By ~ 16

Author(s):

Ivan M. Pchelin ◽

Vasily V. Zlatogursky ◽

Mariya V. Rudneva ◽

Galina A. Chilina ◽

Ali Rezaei-Matehkolaei ◽

...

Keyword(s):

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

28S Rrna ◽

Internal Transcribed Spacer Region ◽

Beta Tubulin ◽

Tubulin Genes

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Systematic Placement of the Enigmatic Southeast Asian Genus Paralamium and an Updated Phylogeny of Tribe Pogostemoneae (Lamiaceae Subfamily Lamioideae)

Frontiers in Plant Science ◽

10.3389/fpls.2021.646133 ◽

2021 ◽

Vol 12 ◽

Author(s):

Fei Zhao ◽

Yi-Wen Wu ◽

Bryan T. Drew ◽

Gang Yao ◽

Ya-Ping Chen ◽

...

Keyword(s):

Chloroplast Dna ◽

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Systematic Position ◽

Monotypic Genus ◽

Internal Transcribed Spacer Region ◽

Generic Level ◽

Phylogenetic Placement ◽

Dichotomous Key ◽

Generic Relationships

Paralamium (Lamiaceae) is a monotypic genus within the subfamily Lamioideae and has a sporadic distribution in subtropical mountains of southeast Asia. Although recent studies have greatly improved our understanding of generic relationships within Lamioideae, the second most species-rich subfamily of Lamiaceae, the systematic position of Paralamium within the subfamily remains unclear. In this study, we investigate the phylogenetic placement of the genus using three datasets: (1) a 69,276 bp plastome alignment of Lamiaceae; (2) a five chloroplast DNA region dataset of tribe Pogostemoneae, and (3) a nuclear ribosomal internal transcribed spacer region dataset of Pogostemoneae. These analyses demonstrate that Paralamium is a member of Pogostemoneae and sister to the monotypic genus Craniotome. In addition, generic-level phylogenetic relationships within Pogostemoneae are also discussed, and a dichotomous key for genera within Pogostemoneae is provided.

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Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region

Genome ◽

10.1139/g97-064 ◽

1997 ◽

Vol 40 (4) ◽

pp. 479-486 ◽

Cited By ~ 19

Author(s):

S. Bekal ◽

J. P. Gauthier ◽

R. Rivoal

Keyword(s):

Phylogenetic Relationships ◽

Internal Transcribed Spacer ◽

Rflp Analysis ◽

Restriction Pattern ◽

Nuclear Ribosomal Dna ◽

Heterodera Avenae ◽

Cyst Nematodes ◽

Internal Transcribed Spacer Region ◽

5.8S Rdna ◽

Pcr Rflp

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establish a molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for each isolate. Relationships between populations were determined from UPGMA analysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H. filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.Key words: cereal cyst nematodes, Heterodera avenae, PCR, RFLP, ribosomal diversity.

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