Evaluation of a novel 16S-23S internal transcribed spacer region PCR and capillary gel electrophoresis method for identification of slowly growing non-tuberculous mycobacteria

2016 ◽  
Author(s):  
Shradha Subedi
Keyword(s):  
Gel Electrophoresis ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Capillary Gel Electrophoresis ◽  
Electrophoresis Method

Development of a polymerase chain reaction and capillary gel electrophoresis method for the detection of chicken or turkey meat in heat-treated pork meat mixtures

2011 ◽  
Vol 708 (1-2) ◽  
pp. 149-154 ◽  
Author(s):  
Juan F. Hernández-Chávez ◽  
Aarón F. González-Córdova ◽  
Roberto Rodríguez-Ramírez ◽  
Belinda Vallejo-Cordoba
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Pork Meat ◽  
Chain Reaction ◽  
Capillary Gel Electrophoresis ◽  
Turkey Meat ◽  
Heat Treated ◽  
Electrophoresis Method ◽  
Polymerase Chain

Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies

2010 ◽  
Vol 31 (3) ◽  
pp. 448-458 ◽  
Author(s):  
Nathan A. Lacher ◽  
Qian Wang ◽  
Rachel K. Roberts ◽  
Heidi J. Holovics ◽  
Serdar Aykent ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Capillary Gel Electrophoresis ◽  
Disulfide Isomer ◽  
Electrophoresis Method

A PRELIMINARY CHARACTERISATION OFSYMBIODINIUMDIVERSITY IN SOME COMMON CORALS FROM SINGAPORE

COSMOS ◽  
2016 ◽  
Vol 12 (01) ◽  
pp. 15-27 ◽  
Author(s):  
JANI THUAIBAH ISA TANZIL ◽  
ABIGAYLE PEK KAYE NG ◽  
YI QING TEY ◽  
BEVERLY HSIN YI TAN ◽  
ERIC YAO YUN ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Internal Transcribed Spacer ◽  
Coral Species ◽  
Elevated Temperatures ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Symbiotic Relationship ◽  
Internal Transcribed Spacer Region ◽  
Porites Lutea ◽  
Gradient Gel Electrophoresis ◽  
Response To Stress

The symbiosis between corals and Symbiodinium dinoflagellates is considered a major driver of the distribution and health of reefs worldwide. This study investigated the genetic identities and diversity of Symbiodinium in seven coral species (Porites lutea, Porites lobata, Acropora millepora, Merulina ampliata, Diploastrea heliopora, Pachyseris speciosa, Pocillopora acuta) from three shallow reefs around Singapore (Kusu Island, Pulau Tekukor, Pulau Satumu). Analyses of 31 colonies using denaturing gradient gel electrophoresis of the nuclear internal transcribed spacer region indicated the dominance of C and D Symbiodinium clades. The latter clade was the predominant symbiont in Pachyseris speciosa collected from Pulau Tekukor but those sampled from Pulau Satumu hosted C27, providing evidence for variable symbiosis in this species. The prevalence of the D clade – noted for their stress tolerance (e.g. to elevated temperatures and sedimentation) – in three of seven coral species examined could underlie the importance of this particular symbiotic relationship for the persistence of Singapore’s impacted reefs. Further characterisation of Symbiodinium communities may provide insights into corals’ response to stress and their bleaching patterns in the future.

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