Abstract
A useful technic for the isolation, separation, and identification of biogenic amines as fluores ent dansyl derivatives is described. A thin-layer chromatographic system is presented for the separation of the dansyl derivatives of phenethylamines from their corresponding β-hydroxyl derivatives, of primary amines from their corresponding N-alkylated derivatives, and of the components present in an extract of mammalian heart. Mass spectrums of dansyl derivatives of various amines and amino acids are presented to illustrate parent ion mass determination. This technic was applied to the identification of various amines present in mammalian heart. Identification was based on cochromatography and identity of parent ion mass with authentic compounds. Piperidine, dimethylamine, phenethylamine, methylamine, phenethanolamine, putrescine, spermidine, spermine, and tyramine were identified.