Differential Surface Plasmon Resonance Imaging for High-Throughput Bioanalyses

2007 ◽  
Vol 79 (2) ◽  
pp. 702-709 ◽  
Author(s):  
Daniel Boecker ◽  
Alexander Zybin ◽  
Vlasta Horvatic ◽  
Christian Grunwald ◽  
Kay Niemax
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

scholarly journals Rapid and Label-Free Immunosensing of Shiga Toxin Subtypes with Surface Plasmon Resonance Imaging

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 280
Author(s):  
Bin Wang ◽  
Bosoon Park ◽  
Jing Chen ◽  
Xiaohua He
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Shiga Toxin ◽  
The United States ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Shiga Toxins

Shiga toxin-producing Escherichia coli (STEC) are responsible for gastrointestinal diseases reported in numerous outbreaks around the world as well as in the United States. Current detection methods have limitation to implement for rapid field-deployable detection with high volume of samples that are needed for regulatory purposes. Surface plasmon resonance imaging (SPRi) has proved to achieve rapid and label-free screening of multiple pathogens simultaneously, so it was evaluated in this work for the detection of Shiga toxins (Stx1a and Stx2a toxoids were used as the less toxic alternatives to Stx1 and Stx2, respectively). Multiple antibodies (Stx1pAb, Stx1-1mAb, Stx1-2mAb, Stx1d-3mAb, Stx1e-4mAb, Stx2pAb, Stx2-1mAb, Stx2-2mAb, and Stx2-10mAb) were spotted one by one by programed microarrayer, on the same high-throughput biochip with 50-nm gold film through multiple crosslinking and blocking steps to improve the orientation of antibodies on the biochip surface. Shiga toxins were detected based on the SPRi signal difference (ΔR) between immobilized testing antibodies and immunoglobulin G (IgG) control. Among the antibodies tested, Stx1pAb showed the highest sensitivity for Stx1 toxoid, with the limit of detection (LOD) of 50 ng/mL and detection time of 20 min. Both Stx2-1mAb and Stx2-2mAb exhibited high sensitivity for Stx2 toxoid. Furthermore, gold nanoparticles (GNPs) were used to amplify the SPRi signals of monoclonal antibodies in a sandwich platform. The LOD reached the level of picogram (pg)/mL with the help of GNP-antibody conjugate. This result proved that SPRi biochip with selected antibodies has the potential for rapid, high-throughput and multiplex detection of Shiga toxins.

Surface Plasmon Resonance Imaging of Polymer Microarrays to Study Protein−Polymer Interactions in High Throughput

Langmuir ◽  
2009 ◽  
Vol 25 (16) ◽  
pp. 9173-9181 ◽  
Author(s):  
Andrew L. Hook ◽  
Helmut Thissen ◽  
Nicolas H. Voelcker
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Protein Polymer ◽  
Polymer Interactions

High-throughput measurement of drug–cyclodextrin kinetic rate constants by a small molecule microarray using surface plasmon resonance imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 3213-3218 ◽  
Author(s):  
Vikramjeet Singh ◽  
Zhuo Li ◽  
Xiaotong Zhou ◽  
Xiaonan Xu ◽  
Jianghui Xu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Small Molecule ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Rate Constants ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Kinetic Rate ◽  
Kinetic Rate Constants

A high-throughput methodology for the measurement of drug–CD kinetic rate constants.

scholarly journals Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology

2017 ◽  
Vol 114 (35) ◽  
pp. E7245-E7254 ◽  
Author(s):  
Shuai Zhao ◽  
Mo Yang ◽  
Wenfei Zhou ◽  
Baichao Zhang ◽  
Zhiqiang Cheng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Dna Mismatch ◽  
Damage Repair ◽  
Capsella Rubella ◽  
Repair Protein

Chemical modifications on histones and DNA/RNA constitute a fundamental mechanism for epigenetic regulation. These modifications often function as docking marks to recruit or stabilize cognate “reader” proteins. So far, a platform for quantitative and high-throughput profiling of the epigenetic interactome is urgently needed but still lacking. Here, we report a 3D-carbene chip-based surface plasmon resonance imaging (SPRi) technology for this purpose. The 3D-carbene chip is suitable for immobilizing versatile biomolecules (e.g., peptides, antibody, DNA/RNA) and features low nonspecific binding, random yet function-retaining immobilization, and robustness for reuses. We systematically profiled binding kinetics of 1,000 histone “reader–mark” pairs on a single 3D-carbene chip and validated two recognition events by calorimetric and structural studies. Notably, a discovery on H3K4me3 recognition by the DNA mismatch repair protein MSH6 in Capsella rubella suggests a mechanism of H3K4me3-mediated DNA damage repair in plant.

High-Throughput Immunophenotyping by Surface Plasmon Resonance Imaging

2007 ◽  
Vol 79 (22) ◽  
pp. 8616-8623 ◽  
Author(s):  
Koichi Kato ◽  
Toshinari Ishimuro ◽  
Yusuke Arima ◽  
Isao Hirata ◽  
Hiroo Iwata
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging
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