scholarly journals Charging YOYO-1 on Capillary Wall for Online DNA Intercalation and Integrating This Approach with Multiplex PCR and Bare Narrow Capillary–Hydrodynamic Chromatography for Online DNA Analysis

2015 ◽  
Vol 87 (3) ◽  
pp. 1518-1522 ◽  
Author(s):  
Huang Chen ◽  
Zaifang Zhu ◽  
Joann Juan Lu ◽  
Shaorong Liu
Keyword(s):  
Multiplex Pcr ◽  
Dna Analysis ◽  
Capillary Wall ◽  
Dna Intercalation ◽  
Hydrodynamic Chromatography ◽  
Narrow Capillary ◽  
Capillary Hydrodynamic

Capillary hydrodynamic chromatography reveals temporal profiles of cell aggregates

2016 ◽  
Vol 910 ◽  
pp. 75-83 ◽  
Author(s):  
Ya-Ru Tang ◽  
Hsin-Yi Huang ◽  
Jie-Bi Hu ◽  
Rajesh Rattinam ◽  
Chun-Hsien Li ◽  
...  
Keyword(s):  
Cell Aggregates ◽  
Hydrodynamic Chromatography ◽  
Temporal Profiles ◽  
Capillary Hydrodynamic

Universal DNA Arrays for High-Throughput SNP Detection of Human Platelet Antigens (HPA)

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4403-4403
Author(s):  
Xin Lin ◽  
Ermelina Enriquez ◽  
Jigar Patel ◽  
Ruth Huang ◽  
Cindi Marks ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Analysis ◽  
Imaging System ◽  
Human Platelet ◽  
Package Insert ◽  
Clinical Samples ◽  
Dna Arrays ◽  
Specific Primer ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Abstract Abstract 4403 Background: DNA analysis of human platelet antigens is known to improve the diagnosis and treatment of neonatal alloimmune thrombocytopenia and minimize the risks of post transfusion purpura and refractoriness to random donor platelet therapy. The availability of reliable molecular platforms such as HPA eMAP assays analyzing HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -11, and -15, are routinely used and eliminate the need for reference sera or large platelet volumes. New generations of HPA assays (HPA eMAP-S Beadchip™ Kit) described here are developed by identification of specific allele in solution and detection of targets by a generic probe array called the Universal BeadChip™ by imaging, HPA-13 was also incorporated in the panel. These assays are easily automatable due to fewer protocol steps. Methods: The BioArray Solutions HPA eMAP-S BeadChip Kit uses the novel Elongation Mediated Multiplexed Analysis of Polymorphisms in Solution (eMAP-S) technology (Lin, X., et al., 2010) to identify the presence or absence of the selected alleles associated with a given phenotype. We amplified 10 DNA fragments covering 12 allele variants which are associated with 12 platelet antigens by using multiplex PCR and HPA gene specific primer pairs. PCR was followed by multiplex allele specific primer extension (ASPE) and Beadchip detection by using the AIS-400 Array Imaging System. To evaluate the overall performance of HPA eMAP-S BeadChip™ Kits, a total 552 clinical samples with known HPA eMAP phenotype and/or serology were analyzed to determine polymorphisms associated with common human platelet antigen (HPA-1, -2, -3, -5, -15) and less common human platelet antigen (HPA-4, -6, -7, -8, -9, -11, -13). For internal study, 176 clinical samples were analyzed at BioArray Solutions (BAS). For the external study, 186 and 190 (total 376) donor samples were tested at two sites and analyzed by BASIS™ software. Phenotype results obtained from the HPA eMAP-S Beadchip™ Kit during the studies were compared to the data generated from the HPA eMAP BeadChip™ Kit and/or serology. Results: For internal and external studies, the concordance between the HPA eMAP-S Beadchip™ kit and HPA eMAP Beadchip™ kit was 99.99%. The discordant call (0.01%) on HPA-1b antigen was investigated. One HPA-1 discordant sample was sent out for sequencing and re-analyzed by using increased amount of DNA from DNA precipitation. The result from sequencing is consistent with the result from HPA eMAP Beadchip™ Kit. The HPA eMAP-S result from higher concentration DNA is also consistent with the result of sequencing. The discordance in the original HPA eMAP-S assay was attributed to less DNA input in the multiplex PCR, which will be corrected in the Package Insert of HPA eMAP-S Beadchip™ Kit. Conclusion: HPA eMAP-S Beadchip™ Kit could be used reliably for the determination of human platelet antigens using DNA analysis. Disclosures: Hashmi: Immucor: Employment.

Direct multiplex PCR for grapevine genotyping and varietal identification

2012 ◽  
Vol 11 (2) ◽  
pp. 182-185 ◽  
Author(s):  
Daniele Migliaro ◽  
Giacomo Morreale ◽  
Massimo Gardiman ◽  
Sara Landolfo ◽  
Manna Crespan
Keyword(s):  
Multiplex Pcr ◽  
Dna Analysis ◽  
Cultivar Identification ◽  
Table Grape ◽  
Plant Tissues ◽  
Grapevine Cultivar ◽  
Research Institutes ◽  
Varietal Identification ◽  
Grape Varieties ◽  
Simple Sequence

Grapevine cultivar identification is based mainly on two complementary methodologies: microsatellite (simple sequence repeat (SSR)) DNA analysis and traditional ampelography. Here, we report a direct multiplex PCR approach that allows the simultaneous amplification of 11 SSR loci from crude samples, i.e. bypassing DNA extraction, by using an engineered DNA polymerase improved to tolerate plant PCR inhibitors. Many different plant tissues were successfully amplified: leaf, root, wood, berry flesh and skin, stalk and must, from wine and table grape varieties, and rootstocks. The direct multiplex PCR that we propose is quicker and cheaper than the methodologies used until now, and provides accurate results. Thus, SSR DNA analysis becomes economically more accessible to a larger number of potential users in addition to research institutes.

An Investigation into Capillary Hydrodynamic Chromatography

1986 ◽  
Vol 3 (4) ◽  
pp. 187-191 ◽  
Author(s):  
Nikolaas C. de Jaeger ◽  
Jan L. Trappers ◽  
Paul Lardon
Keyword(s):  
Hydrodynamic Chromatography ◽  
Capillary Hydrodynamic

Capillary hydrodynamic chromatography

1978 ◽  
Vol 166 (2) ◽  
pp. 373-382 ◽  
Author(s):  
Rodney J. Noel ◽  
Karen M. Gooding ◽  
Fred E. Regnier ◽  
Clyde Orr ◽  
M.E. Mullins
Keyword(s):  
Hydrodynamic Chromatography ◽  
Capillary Hydrodynamic

Particle sizing by capillary hydrodynamic chromatography

1979 ◽  
Vol 5 (1) ◽  
pp. 79-85 ◽  
Author(s):  
Michael E. Mullins ◽  
Clyde Orr
Keyword(s):  
Particle Sizing ◽  
Hydrodynamic Chromatography ◽  
Capillary Hydrodynamic

scholarly journals Particle size analysis by capillary hydrodynamic chromatography(C-HDC).

1990 ◽  
Vol 27 (6) ◽  
pp. 388-393
Author(s):  
Hideo YAMAMOTO ◽  
Tsuyoshi NOMURA
Keyword(s):  
Particle Size ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Hydrodynamic Chromatography ◽  
Capillary Hydrodynamic

scholarly journals Integrated Bare Narrow Capillary-Hydrodynamic Chromatographic System for Free-Solution DNA Separation at the Single-Molecule Level

2013 ◽  
Vol 52 (21) ◽  
pp. 5612-5616 ◽  
Author(s):  
Zaifang Zhu ◽  
Huang Chen ◽  
Wei Wang ◽  
Aaron Morgan ◽  
Congying Gu ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Separation ◽  
Free Solution ◽  
Chromatographic System ◽  
Single Molecule Level ◽  
Narrow Capillary ◽  
Capillary Hydrodynamic

scholarly journals Wide-Bore Capillary Hydrodynamic Chromatography with ICP-MS Detection for Evaluation of Lanthanide Uptake by Molecular Aggregates

2012 ◽  
Vol 28 (4) ◽  
pp. 359 ◽  
Author(s):  
Ryoji UMEHARA ◽  
Hidekazu MIYAHARA ◽  
Akitoshi OKINO ◽  
Makoto HARADA ◽  
Tetsuo OKADA
Keyword(s):  
Molecular Aggregates ◽  
Hydrodynamic Chromatography ◽  
Ms Detection ◽  
Icp Ms ◽  
Capillary Hydrodynamic
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