scholarly journals Charge Manipulation Using Solution and Gas-Phase Chemistry to Facilitate Analysis of Highly Heterogeneous Protein Complexes in Native Mass Spectrometry

2021 ◽  
Vol 93 (7) ◽  
pp. 3337-3342
Author(s):  
Yang Yang ◽  
Chendi Niu ◽  
Cedric E. Bobst ◽  
Igor A. Kaltashov
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Protein Complexes ◽  
Native Mass Spectrometry ◽  
Gas Phase Chemistry ◽  
Phase Chemistry

Mass Spectrometry and Gas-Phase Chemistry of Anilines

ChemInform ◽  
2007 ◽  
Vol 38 (30) ◽  
Author(s):  
Marcos N. Eberlin ◽  
Daniella Vasconcellos Augusti ◽  
Rodinei Augusti
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Gas Phase Chemistry ◽  
Phase Chemistry

scholarly journals Ionic Charge Manipulation Using Solution and Gas-Phase Chemistry to Facilitate Analysis of Highly Heterogeneous Protein Complexes in Native Mass Spectrometry

2020 ◽  
Author(s):  
Yang Yang ◽  
Chendi Niu ◽  
Cedric Bobst ◽  
Igor Kaltashov
Keyword(s):  
Mass Spectra ◽  
Protein Complexes ◽  
Native Mass Spectrometry ◽  
Protein Solutions ◽  
Ionic Charge ◽  
Mass Spectrometers ◽  
New Approach ◽  
Gas Phase Chemistry ◽  
Multiple Charging ◽  
Phase Chemistry

Ionic signal in native MS typically populates high <i>m/z</i> regions of mass spectra, which frequently extend beyond the precursor ion isolation limits of most commercial mass spectrometers. An approach explored in this work relies on adding supercharging reagents to protein solutions as a means of increasing the extent of multiple charging of non-covalent complexes in ESI MS without compromising their integrity. This shifts the ionic signal down the <i>m/z</i> scale to the region where ion selection and isolation can be readily accomplished, followed by limited charge reduction of the isolated ionic population. The feasibility of the new approach is demonstrated using non-covalent complexes formed by hemoglobin with structurally heterogeneous haptoglobin.

Mass Spectrometry and Gas-Phase Chemistry of Supermolecules: A Primer

2012 ◽  
Author(s):  
Egor V. Dzyuba ◽  
Johannes Poppenberg ◽  
Sebastian Richter ◽  
Ralf W. Troff ◽  
Christoph A. Schalley
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Gas Phase Chemistry ◽  
Phase Chemistry
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