Substitution of glutamic acid 109 by aspartic acid alters the substrate specificity and catalytic activity of the .beta.-subunit in the tryptophan synthase bienzyme complex from Salmonella typhimurium

Enzymes Dependent on Pyridoxal Phosphate and Other Carbonyl Compounds As Cofactors ◽

Role of Glutamic Acid 109 in the Active Site of the β Subunit of Tryptophan Synthase from Salmonella Typhimurium in Controlling the Reaction Specificity and Substrate Specificity of the α2β2 Complex

10.1016/b978-0-08-040820-0.50056-x ◽

1991 ◽

pp. 265-267 ◽

Cited By ~ 3

Author(s):

ARVIND M. KAYASTHA ◽

EDITH WILSON MILES

Keyword(s):

Substrate Specificity ◽

Glutamic Acid ◽

Salmonella Typhimurium ◽

Active Site ◽

Tryptophan Synthase ◽

Β Subunit ◽

Reaction Specificity

Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.

10.1016/s0021-9258(20)89492-7 ◽

1991 ◽

Vol 266 (12) ◽

pp. 7618-7625

Author(s):

A M Kayastha ◽

Y Sawa ◽

S Nagata ◽

E W Miles

Keyword(s):

Glutamic Acid ◽

Salmonella Typhimurium ◽

Active Site ◽

Beta Subunit ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Evidence that glutamic acid 49 of tryptophan synthase alpha subunit is a catalytic residue. Inactive mutant proteins substituted at position 49 bind ligands and transmit ligand-dependent to the beta subunit.

10.1016/s0021-9258(18)68348-6 ◽

1988 ◽

Vol 263 (18) ◽

pp. 8611-8614 ◽

Cited By ~ 4

Author(s):

E W Miles ◽

P McPhie ◽

K Yutani

Keyword(s):

Glutamic Acid ◽

Beta Subunit ◽

Alpha Subunit ◽

Catalytic Residue ◽

Tryptophan Synthase ◽

Mutant Proteins

Effects of the phenylalanine-22 .fwdarw. leucine, glutamic acid-49 .fwdarw. methionine, glycine-234 .fwdarw. aspartic acid and glycine-234 .fwdarw. lysine mutations on the folding and stability of the .alpha. subunit of tryptophan synthase from Escherichia coli

Biochemistry ◽

10.1021/bi00358a035 ◽

1986 ◽

Vol 25 (10) ◽

pp. 2965-2974 ◽

Cited By ~ 88

Author(s):

A. M. Beasty ◽

M. R. Hurle ◽

J. T. Manz ◽

T. Stackhouse ◽

J. J. Onuffer ◽

...

Keyword(s):

Escherichia Coli ◽

Glutamic Acid ◽

Aspartic Acid ◽

Alpha Subunit ◽

Identification of a Glutamic Acid and an Aspartic Acid Residue Essential for Catalytic Activity of Aspergillopepsin II, a Non-pepsin Type Acid Proteinase

10.1074/jbc.m910243199 ◽

2000 ◽

Vol 275 (34) ◽

pp. 26607-26614 ◽

Cited By ~ 19

Author(s):

Xiang-Ping Huang ◽

Naofumi Kagami ◽

Hideshi Inoue ◽

Masaki Kojima ◽

Takao Kimura ◽

...

Keyword(s):

Catalytic Activity ◽

Glutamic Acid ◽

Aspartic Acid ◽

Acid Proteinase ◽

Aspartic Acid Residue

High-Affinity Transport of ?-Aminobutyric Acid, Glycine, Taurine, L-Aspartic Acid, and L-Glutamic Acid in Synaptosomal (P2) Tissue: A Kinetic and Substrate Specificity Analysis

Journal of Neurochemistry ◽

10.1111/j.1471-4159.1987.tb05747.x ◽

1987 ◽

Vol 48 (6) ◽

pp. 1851-1856 ◽

Cited By ~ 71

Author(s):

Edmund A. Debler ◽

Abel Lajtha

Keyword(s):

Substrate Specificity ◽

Glutamic Acid ◽

Aspartic Acid ◽

Aminobutyric Acid ◽

High Affinity

DEFINING SUBSTRATE SPECIFICITY IN TRYPTOPHAN SYNTHASE BETA‐SUBUNIT HOMOLOGS

The FASEB Journal ◽

10.1096/fasebj.21.6.a1018-c ◽

2007 ◽

Vol 21 (6) ◽

Author(s):

Santiago Lima ◽

Christopher Schlett ◽

Michael W.W. Adams ◽

Michael Kaufmann ◽

Boris Striepen ◽

...

Keyword(s):

Substrate Specificity ◽

Beta Subunit ◽

Effects of the phenylalanine-22 .fwdarw. leucine, glutamic acid-49 .fwdarw. methionine, glycine-234 .fwdarw. aspartic acid and glycine-234 .fwdarw. lysine mutations on the folding and stability of the .alpha. subunit of tryptophan synthase from Escherichia coli [Erratum to document cited in CA104(23):203041c]

Biochemistry ◽

10.1021/bi00409a064 ◽

1988 ◽

Vol 27 (9) ◽

pp. 3532-3532

Author(s):

A. M. Beasty ◽

M. R. Hurle ◽

J. T. Manz ◽

T. Stackhouse ◽

J. Onuffer ◽

...

Keyword(s):

Escherichia Coli ◽

Glutamic Acid ◽

Aspartic Acid ◽

Alpha Subunit ◽

Heat Capacities from 11 to 305°K, Entropies and Free Energies of Formation of l-Asparagine Monohydrate, l-Aspartic Acid, l-Glutamic Acid, and l-Glutamine

10.1016/s0021-9258(19)67985-8 ◽

1963 ◽

Vol 238 (7) ◽

pp. 2407-2412

Author(s):

John O. Hutchens ◽

Arthur G. Cole ◽

R.A. Robie ◽

J.W. Stout

Keyword(s):

Glutamic Acid ◽

Aspartic Acid ◽

Heat Capacities ◽

Free Energies ◽

Asparagine Monohydrate

Mechanism of mutual activation of the tryptophan synthase alpha and beta subunits. Analysis of the reaction specificity and substrate-induced inactivation of active site and tunnel mutants of the beta subunit.

10.1016/s0021-9258(18)54673-1 ◽

1991 ◽

Vol 266 (32) ◽

pp. 21548-21557 ◽

Cited By ~ 1

Author(s):

S.A. Ahmed ◽

S.B. Ruvinov ◽

A.M. Kayastha ◽

E.W. Miles

Keyword(s):

Active Site ◽

Beta Subunit ◽

Beta Subunits ◽

Tryptophan Synthase ◽

Reaction Specificity