Effects of the phenylalanine-22 .fwdarw. leucine, glutamic acid-49 .fwdarw. methionine, glycine-234 .fwdarw. aspartic acid and glycine-234 .fwdarw. lysine mutations on the folding and stability of the .alpha. subunit of tryptophan synthase from Escherichia coli

Biochemistry ◽  
1986 ◽  
Vol 25 (10) ◽  
pp. 2965-2974 ◽  
Author(s):  
A. M. Beasty ◽  
M. R. Hurle ◽  
J. T. Manz ◽  
T. Stackhouse ◽  
J. J. Onuffer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Alpha Subunit ◽  
Tryptophan Synthase

Interaction of chlorambucil with tRNA

1978 ◽  
Vol 56 (2) ◽  
pp. 132-134 ◽  
Author(s):  
William R. Hunsaker ◽  
A. Fritz Dotzlaf ◽  
Arthur S. Brecher
Keyword(s):  
Escherichia Coli ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Apparent Effect ◽  
Escherichia Coli K12

Preincubation of purified mixed tRNAs from Escherichia coli K12-MO with 2.94 mM chlorambucil (CAB) for 2 h at 37 °C results in the inhibition of the capacity of mixed tRNAs to accept alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, histidine, isoieucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine by 100, 71, 100,100, 100, 95, 32, 88, 36, 26, 96, 78, 44, 31, 34, 98, 38, and 17% respectively. Preincubation of tRNA with 0.75 mM and 0.29 mM CAB inhibited aminoacylation by aspartic acid to the extent of 69 and 17% respectively. CAB has no apparent effect upon the capacity of ATP to function in the formation of aminoacylated tRNALeu.

SOME VITAMIN AND AMINO ACID INTERRELATIONSHIPS IN ESCHERICHIA COLI 113-3: I. THE INHIBITORY EFFECTS OF CYSTINE AND CYSTEINESULPHINIC ACID

1957 ◽  
Vol 3 (7) ◽  
pp. 967-974 ◽  
Author(s):  
J. M. McLaughlan
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Pantothenic Acid ◽  
Inhibitory Effect ◽  
Inhibitory Effects ◽  
Aerobic Conditions ◽  
High Concentrations

Cystine, cysteinesulphinic acid (CSA), and other closely related sulphur-containing amino acids inhibited growth of Escherichia coli 113-3, particularly in aerobic conditions. The cystine inhibition was completely prevented by aspartic acid, partially reversed by pantothenic acid or β-alanine and slightly reversed by lysine or thiamine. The inhibitory effect of CSA was completely or partially reversed by aspartic acid, lysine, glutamic acid, proline, ornithine, or homoserine. Aspartic acid and glutamic acid appeared to reverse the inhibition competitively while lysine seemed to reverse the inhibition in a noncompetitive manner. Reversal of the inhibitory effect of relatively high concentrations of CSA by lysine was not complete, however, unless methionine was also present. Possible mechanisms of the cystine and CSA inhibition are discussed.

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