Oligosaccharides Derived from Bovine Articular Cartilage Keratan Sulfates after Keratanase II Digestion: Implications for Keratan Sulfate Structural Fingerprinting

Bovine articular cartilage fibromodulin has been isolated from animals aged 3 months to 8 years, and the attached keratan sulphate (KS) chains digested with keratanase II. The oligosaccharides generated have been reduced, examined by high-pH anion-exchange chromatography and their structures identified by comparison with standards. It has been shown that in fibromodulin from young articular cartilage, the KS chains do not possess either non-reducing terminal (α2-6)-linked N-acetylneuraminic acid or fucose (α1-3)-linked to sulphated N-acetylglucosamine residues. However, an age-related increase has been observed in the abundance of both (α2-6)-linked N-acetylneuraminic acid and (α1-3)-linked fucose, neither of which is found in KS isolated from non-articular cartilage, irrespective of the age of the source. Interestingly, the KS chain length remains constant as a function of age, which possibly relates to a role in collagen fibril assembly. In addition, no significant age-related changes were identified in levels of galactose sulphation.

Download Full-text

Association of the Aggrecan Keratan Sulfate-rich Region with Collagen in Bovine Articular Cartilage

Journal of Biological Chemistry ◽

10.1074/jbc.274.9.5777 ◽

1999 ◽

Vol 274 (9) ◽

pp. 5777-5781 ◽

Cited By ~ 38

Author(s):

Håkan Hedlund ◽

Erik Hedbom ◽

Dick Heinegård ◽

Silwa Mengarelli-Widholm ◽

Finn P. Reinholt ◽

...

Keyword(s):

Articular Cartilage ◽

Keratan Sulfate ◽

Rich Region ◽

Bovine Articular Cartilage

Download Full-text

Characterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing α(2-3)-linked sialic acid and α(1-3)-linked fucose. A sulphated variant of the VIM-2 epitope

Biochemical Journal ◽

10.1042/bj3190137 ◽

1996 ◽

Vol 319 (1) ◽

pp. 137-141 ◽

Cited By ~ 15

Author(s):

Gavin M. BROWN ◽

Thomas N. HUCKERBY ◽

Beverley L. ABRAM ◽

Ian A. NIEDUSZYNSKI

Keyword(s):

Articular Cartilage ◽

Sialic Acid ◽

Spectroscopic Analysis ◽

Anion Exchange Column ◽

Keratan Sulphate ◽

Bovine Articular Cartilage ◽

Sialyl Lex ◽

Strong Anion Exchange ◽

Keratanase Ii

Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-β-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both α(2-3)-linked sialic acid and α(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAcβ1-] was identified in this study.

Download Full-text

Spheroidal Organoids Reproduce Characteristics of Longitudinal Depth Zones in Bovine Articular Cartilage

Cells Tissues Organs ◽

10.1159/000447532 ◽

2016 ◽

Vol 202 (5-6) ◽

pp. 382-392 ◽

Cited By ~ 2

Author(s):

Shuichi Mizuno ◽

Eiichiro Takada ◽

Naomi Fukai

Keyword(s):

Articular Cartilage ◽

Keratan Sulfate ◽

Cartilage Explants ◽

Collagen Types ◽

Gene Expressions ◽

Bovine Articular Cartilage ◽

3 Dimensional ◽

Cartilage Development ◽

Matrix Metalloproteinase 13 ◽

Dimensional Cell

Articular cartilage has multiple histologically distinct longitudinal depth zones. Development and pathogenesis occur throughout these zones. Cartilage explants, monolayer cell culture and reconstituted 3-dimensional cell constructs have been used for investigating mechanisms of pathophysiology in articular cartilage. Such models have been insufficient to reproduce zone-dependent cellular characteristics and extracellular matrix (ECM) upon investigation into cartilage development and pathogenesis. Therefore, we defined a chondrocyte spheroid model consistently formed with isolated chondrocytes from longitudinal depth zones without extrinsic materials. This spheroid showed zone-dependent characteristics of size, cartilage-specific ECM (collagen types I and II, aggrecan and keratan sulfate) and gene expressions of anabolic and catabolic molecules (matrix molecules and matrix metalloproteinase-13). In addition, the spheroid model is small enough to maintain the viability of cells and point symmetry to analyze the gradient of diffusive molecules. This spheroid organoid model will be useful to elucidate the mechanism of histogenesis and pathogenesis in articular cartilage.

Download Full-text