Pathway for uncoupler-induced calcium efflux in rat liver mitochondria: inhibition by Ruthenium Red

Biochemistry ◽  
1984 ◽  
Vol 23 (8) ◽  
pp. 1645-1651 ◽  
Author(s):  
Paolo Bernardi ◽  
Venturina Paradisi ◽  
Tullio Pozzan ◽  
Giovanni Felice Azzone
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux

scholarly journals The action of Nupercaine on calcium efflux from rat liver mitochondria

1980 ◽  
Vol 188 (3) ◽  
pp. 749-755 ◽  
Author(s):  
A P Dawson ◽  
D V Fulton
Keyword(s):  
Steady State ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux

1. Nupercaine inhibits the Ca2+ efflux from rat liver mitochondria observed in the presence of Ruthenium Red, 50% inhibition being obtained at 80 microM-Nupercaine. 2. Neither the Ruthenium Red-stimulated efflux nor its inhibition by Nupercaine can be directly attributed to effects on mitochondrial stability. 3. Nupercaine perturbs the steady-state external Ca2+ concentration in the absence of Ruthenium Red to an extent that is explicable in terms of the inhibition of Ca2+ efflux. 4. Various factors that are likely to be involved in determining steady-state extra-mitochondrial Ca2+ concentrations are discussed.

Ruthenium red-insensitive calcium efflux from rat liver mitochondria results from two distinct processes

Cell Calcium ◽  
1984 ◽  
Vol 5 (3) ◽  
pp. 283
Author(s):  
Günter Blaich ◽  
Herbert Krell ◽  
Erich Pfaff
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux

scholarly journals Effect of ruthenium red on calcium efflux from rat liver mitochondria

FEBS Letters ◽  
1980 ◽  
Vol 120 (2) ◽  
pp. 255-258 ◽  
Author(s):  
F. Rigoni ◽  
Y. Mathien-Shire ◽  
R. Deana
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux

Induction of calcium efflux from isolated rat-liver mitochondria by 1,2-dibromoethane

1986 ◽  
Vol 852 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Alberto Masini ◽  
Barbara Botti ◽  
Daniela Ceccarelli ◽  
Umberto Muscatello ◽  
Vanio Vannini
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux ◽  
Isolated Rat Liver ◽  
Isolated Rat

scholarly journals Effect of micromolar concentrations of manganese ions on calcium-ion cycling in rat liver mitochondria

1983 ◽  
Vol 212 (3) ◽  
pp. 773-782 ◽  
Author(s):  
B P Hughes ◽  
J H Exton
Keyword(s):  
Rat Liver ◽  
Calcium Ion ◽  
Incubation Medium ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Manganese Ions ◽  
Dose Dependent Decrease ◽  
Incubation Temperatures ◽  
Dose Dependent

The effects of micromolar concentrations of Mn2+ on the rat liver mitochondrial Ca2+ cycle were investigated. It was found that the addition of Mn2+ to mitochondria which were cycling 45Ca2+ led to a rapid dose dependent decrease in the concentration of extramitochondrial 45Ca2+ of about 1 nmol/mg of protein. The effect was complete within 30 s, was half maximal with 10 microM Mn2+ and was observed in the presence of 3 mM Mg2+ and 1 mM ATP. It occurred over a broad range of incubation temperatures, pH and mitochondrial Ca2+ loads. It was not observed when either Mg2+ or phosphate was absent from the incubation medium, or in the presence of Ruthenium Red. These findings indicate that micromolar concentrations of Mn2+ stimulate the uptake of Ca2+ by rat liver mitochondria, and provide evidence for an interaction between Mg2+ and Mn2+ in the control of mitochondrial Ca2+ cycling.

The inhibition of calcium efflux from rat liver mitochondria by halogenated anesthetics

1988 ◽  
Vol 155 (2) ◽  
pp. 978-983 ◽  
Author(s):  
D. Branca ◽  
M.L. Varotto ◽  
E. Vincenti ◽  
G. Scutari
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Calcium Efflux

On the nature of ruthenium red (RR)-prevented destructive calcium release from isolated rat liver mitochondria

Cell Calcium ◽  
1984 ◽  
Vol 5 (3) ◽  
pp. 289
Author(s):  
Herbert Krell ◽  
Günter Blaich ◽  
Erich Pfaff
Keyword(s):  
Rat Liver ◽  
Calcium Release ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat

scholarly journals Stimulation of the respiration rate of rat liver mitochondria by sub-micromolar concentrations of extramitochondrial Ca2+

1987 ◽  
Vol 245 (1) ◽  
pp. 217-222 ◽  
Author(s):  
J D Johnston ◽  
M D Brand
Keyword(s):  
Respiration Rate ◽  
Rat Liver ◽  
Oxidative Metabolism ◽  
Mitochondrial Respiration ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria ◽  
Mitochondrial Matrix ◽  
Stimulation Of

1. The respiration rate of rat liver mitochondria was stimulated by up to 70% when the extramitochondrial Ca2+ concentration was raised from 103 to 820 nM. This occurred when pyruvate, 2-oxoglutarate, or threo-(Ds)-isocitrate was employed as substrate, but not when succinate was used. 2. Ruthenium Red prevented the stimulation of mitochondrial respiration by extramitochondrial Ca2+, showing that the effect required Ca2+ uptake into the mitochondrial matrix. 3. Starvation of rats for 48 h abolished the stimulation of mitochondrial respiration by extramitochondrial Ca2+ when pyruvate was used as substrate, but did not affect the stimulation of 2-oxoglutarate oxidation by extramitochondrial Ca2+. 4. Our findings are in accord with proposals that oxidative metabolism in liver mitochondria may be stimulated by Ca2+ activation of intramitochondrial dehydrogenases.

Ruthenium red as a carrier of electrons between external NADH and cytochrome C in rat liver mitochondria

1976 ◽  
Vol 69 (3) ◽  
pp. 812-815 ◽  
Author(s):  
Klaus Schwerzmann ◽  
Paolo Gazzotti ◽  
Ernesto Carafoli
Keyword(s):  
Cytochrome C ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Ruthenium Red ◽  
Rat Liver Mitochondria
Sign in / Sign up

Export Citation Format

Share Document