Phenylalanine transfer ribonucleic acid from Escherichia coli B, isolation and characterization of oligonucleotides from ribonuclease T1 and ribonuclease A hydrolyzates

Biochemistry ◽  
1969 ◽  
Vol 8 (4) ◽  
pp. 1643-1655 ◽  
Author(s):  
Mayo Uziel ◽  
H. G. Gassen
Keyword(s):  
Escherichia Coli ◽  
Ribonucleic Acid ◽  
Ribonuclease A ◽  
Ribonuclease T1 ◽  
Isolation And Characterization ◽  
Escherichia Coli B

scholarly journals The nucleotide sequences of some large ribonuclease T1 products from bacteriophage R17 ribonucleic acid

1971 ◽  
Vol 124 (2) ◽  
pp. 357-366 ◽  
Author(s):  
Peter G. N. Jeppesen
Keyword(s):  
Escherichia Coli ◽  
Thin Layer ◽  
Ribonucleic Acid ◽  
Chromatographic Separation ◽  
Nucleotide Sequences ◽  
Two Dimensional ◽  
Ribonuclease T1 ◽  
Unique Pattern ◽  
Fingerprinting Technique

A method of ‘fingerprinting’ high-molecular-weight32P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T1 digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The ‘fingerprinting’ technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences.

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