The Pathogenic Human Sulfite Oxidase Mutants G473D and A208D Are Defective in Intramolecular Electron Transfer†

Biochemistry ◽  
2005 ◽  
Vol 44 (42) ◽  
pp. 13734-13743 ◽  
Author(s):  
Changjian Feng ◽  
Heather L. Wilson ◽  
Gordon Tollin ◽  
Andrei V. Astashkin ◽  
James T. Hazzard ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase

Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

2019 ◽  
Vol 476 (12) ◽  
pp. 1805-1815 ◽  
Author(s):  
Daniel Bender ◽  
Alexander Tobias Kaczmarek ◽  
Dimitri Niks ◽  
Russ Hille ◽  
Guenter Schwarz
Keyword(s):  
Electron Transfer ◽  
Spectroscopic Studies ◽  
Sulfite Oxidase ◽  
Nitrite Reduction ◽  
Intramolecular Electron Transfer ◽  
Intermembrane Space ◽  
Electron Transfer Mechanism ◽  
Physiological Relevance ◽  
Order Of Magnitude ◽  
Human Sulfite Oxidase

AbstractIn addition to nitric oxide (NO) synthases, molybdenum-dependent enzymes have been reported to reduce nitrite to produce NO. Here, we report the stoichiometric reduction in nitrite to NO by human sulfite oxidase (SO), a mitochondrial intermembrane space enzyme primarily involved in cysteine catabolism. Kinetic and spectroscopic studies provide evidence for direct nitrite coordination at the molybdenum center followed by an inner shell electron transfer mechanism. In the presence of the physiological electron acceptor cytochrome c, we were able to close the catalytic cycle of sulfite-dependent nitrite reduction thus leading to steady-state NO synthesis, a finding that strongly supports a physiological relevance of SO-dependent NO formation. By engineering SO variants with reduced intramolecular electron transfer rate, we were able to increase NO generation efficacy by one order of magnitude, providing a mechanistic tool to tune NO synthesis by SO.

scholarly journals Effects of Interdomain Tether Length and Flexibility on the Kinetics of Intramolecular Electron Transfer in Human Sulfite Oxidase

Biochemistry ◽  
2010 ◽  
Vol 49 (6) ◽  
pp. 1290-1296 ◽  
Author(s):  
Kayunta Johnson-Winters ◽  
Anna R. Nordstrom ◽  
Safia Emesh ◽  
Andrei V. Astashkin ◽  
Asha Rajapakshe ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase ◽  
Kinetics Of

Essential Role of Conserved Arginine 160 in Intramolecular Electron Transfer in Human Sulfite Oxidase†

Biochemistry ◽  
2003 ◽  
Vol 42 (42) ◽  
pp. 12235-12242 ◽  
Author(s):  
Changjian Feng ◽  
Heather L. Wilson ◽  
John K. Hurley ◽  
James T. Hazzard ◽  
Gordon Tollin ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Essential Role ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase

scholarly journals Role of Conserved Tyrosine 343 in Intramolecular Electron Transfer in Human Sulfite Oxidase

2002 ◽  
Vol 278 (5) ◽  
pp. 2913-2920 ◽  
Author(s):  
Changjian Feng ◽  
Heather L. Wilson ◽  
John K. Hurley ◽  
James T. Hazzard ◽  
Gordon Tollin ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase ◽  
Conserved Tyrosine

Probing the role of a conserved salt bridge in the intramolecular electron transfer kinetics of human sulfite oxidase

2013 ◽  
Vol 18 (6) ◽  
pp. 645-653 ◽  
Author(s):  
Kayunta Johnson-Winters ◽  
Amanda C. Davis ◽  
Anna R. Arnold ◽  
Robert E. Berry ◽  
Gordon Tollin ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Salt Bridge ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Electron Transfer Kinetics ◽  
Human Sulfite Oxidase ◽  
Kinetics Of

Effects of mutating aromatic surface residues of the heme domain of human sulfite oxidase on its heme midpoint potential, intramolecular electron transfer, and steady-state kinetics

2013 ◽  
Vol 42 (9) ◽  
pp. 3043-3049 ◽  
Author(s):  
Amanda C. Davis ◽  
Matthew J. Cornelison ◽  
Kimberly T. Meyers ◽  
Asha Rajapakshe ◽  
Robert E. Berry ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Steady State ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Midpoint Potential ◽  
Steady State Kinetics ◽  
Heme Domain ◽  
Human Sulfite Oxidase

Intramolecular electron transfer controls nitrite reduction in molybdenum-containing sulfite oxidase

Nitric Oxide ◽  
2014 ◽  
Vol 42 ◽  
pp. 113
Author(s):  
Guenter Schwarz ◽  
Sabina Krizowski ◽  
Jun Wang ◽  
Dimitri Niks ◽  
Courtney Sparacino-Watkins ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Nitrite Reduction ◽  
Intramolecular Electron Transfer

The pH dependence of intramolecular electron transfer rates in sulfite oxidase at high and low anion concentrations

1999 ◽  
Vol 4 (4) ◽  
pp. 390-401 ◽  
Author(s):  
Andrew Pacheco ◽  
James T. Hazzard ◽  
G. Tollin ◽  
J. H. Enemark
Keyword(s):  
Electron Transfer ◽  
Ph Dependence ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Transfer Rates

Chicken liver sulfite oxidase. Kinetics of reduction by laser-photoreduced flavins and intramolecular electron transfer

Biochemistry ◽  
1988 ◽  
Vol 27 (8) ◽  
pp. 2918-2926 ◽  
Author(s):  
Cary A. Kipke ◽  
Michael A. Cusanovich ◽  
Gordon Tollin ◽  
Roger A. Sunde ◽  
John H. Enemark
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Chicken Liver ◽  
Intramolecular Electron Transfer ◽  
Kinetics Of ◽  
Kinetics Of Reduction

Effect of Solution Viscosity on Intramolecular Electron Transfer in Sulfite Oxidase†

Biochemistry ◽  
2002 ◽  
Vol 41 (18) ◽  
pp. 5816-5821 ◽  
Author(s):  
Changjian Feng ◽  
Rohit V. Kedia ◽  
James T. Hazzard ◽  
John K. Hurley ◽  
Gordon Tollin ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Solution Viscosity ◽  
Intramolecular Electron Transfer
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