Evaluation of Microbeads of Calcium Alginate as a Fluidized Bed Medium for Affinity Chromatography of Aspergillus niger Pectinase

2004 ◽  
Vol 20 (5) ◽  
pp. 1490-1495 ◽  
Author(s):  
I. Roy ◽  
S. Jain ◽  
S. Teotia ◽  
M.N. Gupta
Keyword(s):  
Aspergillus Niger ◽  
Affinity Chromatography ◽  
Fluidized Bed ◽  
Calcium Alginate

Isolation and purification of endo-polygalacturonase by affinity chromatography in a fluidized bed reactor

1989 ◽  
Vol 40 (1) ◽  
pp. B7-B19 ◽  
Author(s):  
W. Somers ◽  
K. Van 't Reit ◽  
H. Rozie ◽  
F.M. Rombouts ◽  
J. Visser
Keyword(s):  
Affinity Chromatography ◽  
Fluidized Bed ◽  
Fluidized Bed Reactor ◽  
Isolation And Purification

scholarly journals A simple method of affinity chromatography for the purification of glucoamylase obtained from Aspergillus niger C

FEBS Letters ◽  
1982 ◽  
Vol 149 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Andrzej Paszczyński ◽  
Irena Miedziak ◽  
Jerzy Łobarzewski ◽  
Janina Kochmańska ◽  
Jerzy Trojanowski
Keyword(s):  
Aspergillus Niger ◽  
Affinity Chromatography ◽  
Simple Method

Direct recovery of malate dehydrogenase from highly turbid yeast cell homogenate using dye-ligand affinity chromatography in stirred fluidized bed

2018 ◽  
Vol 1100-1101 ◽  
pp. 65-75 ◽  
Author(s):  
Kuei-Hsiang Chen ◽  
Sze-Ying Lee ◽  
Pau-Loke Show ◽  
Shih-Cheng Hong ◽  
Yu-Kaung Chang
Keyword(s):  
Affinity Chromatography ◽  
Yeast Cell ◽  
Fluidized Bed ◽  
Malate Dehydrogenase ◽  
Ligand Affinity ◽  
Cell Homogenate

scholarly journals Amylase Production by Aspergillus niger immobilized in Microporous Calcium Alginate Gel Beads

2021 ◽  
Vol 26 (4) ◽  
pp. 2825-2833
Author(s):  
UCHENNA OYEAGU ◽  
CHARLES NWUCHE ◽  
CHRISTIAN OGBONNA ◽  
JAMES OGBONNA
Keyword(s):  
Aspergillus Niger ◽  
Calcium Chloride ◽  
Calcium Alginate ◽  
Chloride Concentration ◽  
Alpha Amylase ◽  
Alginate Gel ◽  
Gel Beads ◽  
Amylase Production ◽  
Calcium Alginate Gel ◽  
Bead Diameter

Microporous calcium alginate gel beads were investigated as potential solutions to mass transfer limitations in immobilized cultures. The beads were prepared by gelling mixtures of sodium alginate and fillers (starch or granulated sugars) in calcium chloride solution. The resulting beads were cured in the same solution, during which the fillers leached out of the beads thereby creating micro-pores in the beads (microporous beads). The effectiveness of the microporous beads in improving amylase production by Aspergillus niger LC 269109 was investigated. Spores of A. niger were immobilized in the microporous beads and used for batch alpha amylase and gluco amylase production. Amylase production by the A. niger immobilized in the microporous beads were significantly higher (p<0.01) than the values obtained with the conventional calcium alginate gel beads. Under all the conditions investigated, gluco-amylase activities were significantly (p<0.01) higher than the alpha-amylase activities. Under the optimum conditions of inoculum concentration (1.0 × 105 spores/ml), pH (6), temperature (35°C), bead diameter (3 mm) and calcium chloride concentration (1.5%), the gluco-amylase and alpha amylase activities were 11.98 U/ml and 6.7 U/ml respectively, which were significantly higher (p<0.05) than the 7.8 U/ml and 3.2 U/ml obtained with the conventional gel beads.

Application of fluidized bed reactor containing GOx/MnFe2O4/calcium alginate nano-composite in degradation of a model pollutant

2018 ◽  
Vol 6 (5) ◽  
pp. 6414-6420 ◽  
Author(s):  
Pezhman Zolfaghari ◽  
Rahim Shojaat ◽  
Afzal Karimi ◽  
Naghi Saadatjoo
Keyword(s):  
Fluidized Bed ◽  
Calcium Alginate ◽  
Fluidized Bed Reactor ◽  
Nano Composite
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