scholarly journals Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies

2013 ◽  
Vol 135 (47) ◽  
pp. 17793-17803 ◽  
Author(s):  
Yun Han ◽  
Guangjin Hou ◽  
Christopher L. Suiter ◽  
Jinwoo Ahn ◽  
In-Ja L. Byeon ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Structural Plasticity ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Sequence Dependent ◽  
Angle Spinning ◽  
Hiv 1

scholarly journals 19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies

2019 ◽  
Vol 141 (14) ◽  
pp. 5681-5691 ◽  
Author(s):  
Manman Lu ◽  
Mingzhang Wang ◽  
Ivan V. Sergeyev ◽  
Caitlin M. Quinn ◽  
Jochem Struppe ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Angle Spinning ◽  
Fast Magic Angle Spinning ◽  
Hiv 1

scholarly journals Fast Magic-Angle Spinning 19 F NMR Spectroscopy of HIV-1 Capsid Protein Assemblies

2018 ◽  
Vol 57 (50) ◽  
pp. 16375-16379 ◽  
Author(s):  
Mingzhang Wang ◽  
Manman Lu ◽  
Matthew P. Fritz ◽  
Caitlin M. Quinn ◽  
In-Ja L. Byeon ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Capsid Protein ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Angle Spinning ◽  
Fast Magic Angle Spinning ◽  
Hiv 1 ◽  
19 F Nmr

scholarly journals Fast Magic-Angle Spinning 19 F NMR Spectroscopy of HIV-1 Capsid Protein Assemblies

2018 ◽  
Vol 130 (50) ◽  
pp. 16613-16617 ◽  
Author(s):  
Mingzhang Wang ◽  
Manman Lu ◽  
Matthew P. Fritz ◽  
Caitlin M. Quinn ◽  
In-Ja L. Byeon ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Capsid Protein ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Angle Spinning ◽  
Fast Magic Angle Spinning ◽  
Hiv 1 ◽  
19 F Nmr

scholarly journals Dynamic regulation of HIV-1 capsid interaction with the restriction factor TRIM5α identified by magic-angle spinning NMR and molecular dynamics simulations

2018 ◽  
Vol 115 (45) ◽  
pp. 11519-11524 ◽  
Author(s):  
Caitlin M. Quinn ◽  
Mingzhang Wang ◽  
Matthew P. Fritz ◽  
Brent Runge ◽  
Jinwoo Ahn ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Building Blocks ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Capsid Assembly ◽  
Dynamic Regulation ◽  
Angle Spinning ◽  
Dynamics Simulations ◽  
Hiv 1

The host factor protein TRIM5α plays an important role in restricting the host range of HIV-1, interfering with the integrity of the HIV-1 capsid. TRIM5 triggers an antiviral innate immune response by functioning as a capsid pattern recognition receptor, although the precise mechanism by which the restriction is imposed is not completely understood. Here we used an integrated magic-angle spinning nuclear magnetic resonance and molecular dynamics simulations approach to characterize, at atomic resolution, the dynamics of the capsid’s hexameric and pentameric building blocks, and the interactions with TRIM5α in the assembled capsid. Our data indicate that assemblies in the presence of the pentameric subunits are more rigid on the microsecond to millisecond timescales than tubes containing only hexamers. This feature may be of key importance for controlling the capsid’s morphology and stability. In addition, we found that TRIM5α binding to capsid induces global rigidification and perturbs key intermolecular interfaces essential for higher-order capsid assembly, with structural and dynamic changes occurring throughout the entire CA polypeptide chain in the assembly, rather than being limited to a specific protein-protein interface. Taken together, our results suggest that TRIM5α uses several mechanisms to destabilize the capsid lattice, ultimately inducing its disassembly. Our findings add to a growing body of work indicating that dynamic allostery plays a pivotal role in capsid assembly and HIV-1 infectivity.

scholarly journals Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR

2020 ◽  
Vol 27 (9) ◽  
pp. 863-869 ◽  
Author(s):  
Manman Lu ◽  
Ryan W. Russell ◽  
Alexander J. Bryer ◽  
Caitlin M. Quinn ◽  
Guangjin Hou ◽  
...  
Keyword(s):  
Magic Angle Spinning ◽  
Atomic Resolution ◽  
Magic Angle ◽  
Angle Spinning ◽  
Hiv 1 ◽  
Resolution Structure

Magic Angle Spinning NMR Studies of Protein Assemblies: Recent Advances in Methodology and Applications

2013 ◽  
pp. 293-357 ◽  
Author(s):  
Guangjin Hou ◽  
Christopher L. Suiter ◽  
Si Yan ◽  
Huilan Zhang ◽  
Tatyana Polenova
Keyword(s):  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Nmr Studies ◽  
Recent Advances ◽  
Angle Spinning

scholarly journals Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy

2013 ◽  
Vol 46 (9) ◽  
pp. 2047-2058 ◽  
Author(s):  
Si Yan ◽  
Christopher L. Suiter ◽  
Guangjin Hou ◽  
Huilan Zhang ◽  
Tatyana Polenova
Keyword(s):  
Nmr Spectroscopy ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Assemblies ◽  
Structure And Dynamics ◽  
Angle Spinning
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