A Cascade Signaling Network between Artificial Cells Switching Activity of Synthetic Transmembrane Channels

2020 ◽  
Author(s):  
Qiuxia Yang ◽  
Zhenzhen Guo ◽  
Hui Liu ◽  
Ruizi Peng ◽  
Liujun Xu ◽  
...  
Keyword(s):  
Signaling Network ◽  
Switching Activity ◽  
Artificial Cells ◽  
Transmembrane Channels

Programmable Aggregation of Artificial Cells with DNA Signals

2020 ◽  
Author(s):  
Hengming Qiu ◽  
Feiran Li ◽  
Yancheng Du ◽  
Ruixin Li ◽  
Ji Yeon Hyun ◽  
...  
Keyword(s):  
Cell Aggregation ◽  
Cell System ◽  
Cell Physiology ◽  
Artificial Cells ◽  
Intracellular Enzyme ◽  
Dna Oligonucleotides ◽  
Synthetic Cells ◽  
Transmembrane Channels ◽  
Communication And Coordination ◽  
And Migration

ABSTRACTCell aggregation is a complex behavior, which is closely related to the viability, differentiation, and migration of cells. An effort to create synthetic analogs could lead to considerable advances in cell physiology and biophysics. Rendering and modulating such a dynamic artificial cell system require mechanisms for receiving, transducing, and transmitting intercellular signals, yet effective tools are limited at present. Here we construct synthetic cells from engineered lipids and show their programmable aggregation behaviors using DNA oligonucleotides as a signaling molecule. The artificial cells have transmembrane channels made of DNA origami that are used to recognize and process intercellular signals. We demonstrate that multiple small vesicles aggregate onto a giant vesicle after a transduction of external DNA signals by an intracellular enzyme, and that the small vesicles dissociate when receiving ‘release’ signals. This work provides new possibilities for building synthetic protocells capable of chemical communication and coordination.

Artificial cells containing sustainable energy conversion engines

2019 ◽  
Vol 3 (5) ◽  
pp. 573-578 ◽  
Author(s):  
Kwanwoo Shin
Keyword(s):  
Energy Conversion ◽  
Nicotinamide Adenine Dinucleotide ◽  
Sustainable Energy ◽  
Living Cells ◽  
Energy Transduction ◽  
Artificial Cells ◽  
Energy Conversion Process ◽  
Metabolic Reactions ◽  
Metabolic Activities ◽  
Reduced Nicotinamide Adenine Dinucleotide

Living cells naturally maintain a variety of metabolic reactions via energy conversion mechanisms that are coupled to proton transfer across cell membranes, thereby producing energy-rich compounds. Until now, researchers have been unable to maintain continuous biochemical reactions in artificially engineered cells, mainly due to the lack of mechanisms that generate energy-rich resources, such as adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide (NADH). If these metabolic activities in artificial cells are to be sustained, reliable energy transduction strategies must be realized. In this perspective, this article discusses the development of an artificially engineered cell containing a sustainable energy conversion process.

Chemosensory cholinergic signaling network in the thymic medullary epithelium

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
A Soultanova ◽  
A Panneck ◽  
A Rafiq ◽  
B Schütz ◽  
V Chubanov ◽  
...  
Keyword(s):  
Signaling Network ◽  
Cholinergic Signaling

Chemosensory cholinergic signaling network in the thymic medullary epithelium

Pneumologie ◽  
2016 ◽  
Vol 70 (07) ◽  
Author(s):  
A Soultanova ◽  
C Cen ◽  
K Fleck ◽  
G Krasteva-Christ ◽  
U Boehm ◽  
...  
Keyword(s):  
Signaling Network ◽  
Cholinergic Signaling

Artificial cells, cell engineering and therapy

2007 ◽  
Author(s):  
S. Prakash
Keyword(s):  
Cell Engineering ◽  
Artificial Cells

Power Optimization of Sequential Circuits Using Switching Activity Based Clock Gating

2010 ◽  
Vol E93-A (12) ◽  
pp. 2472-2480 ◽  
Author(s):  
Xin MAN ◽  
Takashi HORIYAMA ◽  
Shinji KIMURA
Keyword(s):  
Power Optimization ◽  
Sequential Circuits ◽  
Switching Activity ◽  
Clock Gating
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