Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Chemical Tools in Live Cells

2017 ◽  
Vol 139 (10) ◽  
pp. 3651-3662 ◽  
Author(s):  
Song-Yi Lee ◽  
Myeong-Gyun Kang ◽  
Sanghee Shin ◽  
Chulhwan Kwak ◽  
Taejoon Kwon ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Live Cells ◽  
Inner Mitochondrial Membrane ◽  
Membrane Proteome ◽  
Chemical Tools

scholarly journals Light-inducible Deformation of Mitochondria in Live Cells

2020 ◽  
Author(s):  
Yutong Song ◽  
Peiyuan Huang ◽  
Xiaoying Liu ◽  
Bianxiao Cui ◽  
Liting Duan
Keyword(s):  
Outer Membrane ◽  
Molecular Motors ◽  
Optical Method ◽  
Mitochondrial Membrane ◽  
Motor Proteins ◽  
Morphological Changes ◽  
Live Cells ◽  
Mechanical Forces ◽  
Outer Mitochondrial Membrane ◽  
Inner Mitochondrial Membrane

AbstractMitochondria, the powerhouse of the cell, are dynamic organelles that undergo constant morphological changes. Increasing evidence indicates that mitochondria morphologies and functions can be modulated by mechanical cues. However, the mechano-sensing and -responding properties of mitochondria and the correlation between mitochondrial morphologies and functions are unclear due to the lack of methods to precisely exert mechano-stimulation on and deform mitochondria inside live cells. Here we present an optogenetic approach that uses light to induce deformation of mitochondria by recruiting molecular motors to the outer mitochondrial membrane via light-activated protein-protein hetero-dimerization. Mechanical forces generated by motor proteins distort the outer membrane, during which the inner mitochondrial membrane can also be deformed. Moreover, this optical method can achieve subcellular spatial precision and be combined with other optical dimerizers and molecular motors. This method presents a novel mitochondria-specific mechano-stimulator for studying mitochondria mechanobiology and the interplay between mitochondria shapes and functions.

Modulation of the calcium-activated BK-channel of the inner mitochondrial membrane by hypoxia

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
D Siemen ◽  
Y Cheng ◽  
X Gu ◽  
P Bednarczyk ◽  
GG Haddad ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Bk Channel ◽  
Inner Mitochondrial Membrane

The Efects of Spaceflight on Mitochondria in Human Lymphocytes (Jurkat).

1999 ◽  
Vol 5 (S2) ◽  
pp. 1118-1119
Author(s):  
Heide Schatten ◽  
Marian Lewis
Keyword(s):  
Mitochondrial Membrane ◽  
Space Shuttle ◽  
Human Lymphocytes ◽  
Jurkat Cells ◽  
Apoptotic Bodies ◽  
Inner Mitochondrial Membrane ◽  
Mitochondrial Alterations ◽  
Mitochondrial Volume ◽  
Number Of Cells ◽  
Related Increase

Spaceflight induced mitochondrial alterations have been reported for muscle and may be associated with altered physiological functions in space. Mitochondrial alterations are also indicative of preapoptotic events which are seen in greater amounts in cells exposed to spaceflight when compared with cells cultured at 1 g. Preapoptotic mitochondrial changes include alterations of processes at the inner mitochondrial membrane and can result in changes in mitochondrial volume. Higher amounts of oxidative stress during space flight may be one of the causes for changes which lead to apoptosis. Jurkat cells flown on the STS-76 space shuttle mission showed an increase in the number of cells with apoptotic bodies early in the mission and a time-dependent, microgravity-related increase in the Fas/APO-1 cell death factor. Here we investigated the morphology of mitochondria in Jurkat cells exposed to spaceflight during the STS-76 mission.

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