Determination of the Translational and Rotational Diffusion Coefficients of Rodlike Particles Using Depolarized Dynamic Light Scattering

Langmuir ◽  
2000 ◽  
Vol 16 (4) ◽  
pp. 1689-1695 ◽  
Author(s):  
Dieter Lehner ◽  
Helmut Lindner ◽  
Otto Glatter
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Diffusion Coefficients ◽  
Rotational Diffusion ◽  
Translational And Rotational Diffusion ◽  
Rodlike Particles ◽  
Rotational Diffusion Coefficients

Translational and Rotational Diffusion Coefficients of Gold Nanorods Dispersed in Mixtures of Water and Glycerol by Polarized Dynamic Light Scattering

2019 ◽  
Vol 123 (44) ◽  
pp. 9491-9502 ◽  
Author(s):  
Francisco E. Berger Bioucas ◽  
Cornelia Damm ◽  
Wolfgang Peukert ◽  
Michael H. Rausch ◽  
Thomas M. Koller ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Gold Nanorods ◽  
Diffusion Coefficients ◽  
Rotational Diffusion ◽  
Translational And Rotational Diffusion ◽  
Rotational Diffusion Coefficients

scholarly journals Size and Shape of Human Fibrinogen in Solution

1977 ◽  
Author(s):  
V. Hofmann ◽  
P.W. Straub ◽  
T. Binkert ◽  
E. Serrallach ◽  
W. Känzig ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Analytical Ultracentrifugation ◽  
Rotational Diffusion ◽  
Axial Ratio ◽  
Major Axis ◽  
Fluorescence Depolarization ◽  
Human Fibrinogen ◽  
Size And Shape ◽  
Rotational Diffusion Coefficients

In order to obtain information on size and shape of the fibrinogen molecule in solution the translational diffusion coefficient (DT), the rotational diffusion coefficients (DR⊥ and DR//) and the sedimentation coefficients (S) have been measured on human fibrinogen with a clottaoility above 95%. The methods used were dynamic light scattering, nanosecond fluorescence depolarization and analytical ultracentrifugation. Dynamic light scattering yields DT = 2.0 ± 3% x 10-7 cm2sec–1 at a concentration of 7 mg/ml in 0.15 M Tris-NaCl, pH 7.4. DT is strongly dependent on concentration, being 3.4 ± 10% × 10-7 cm2 sec-1 at 0.1 mg/ml. The rotation along the minor axis as measured with the same method is DR = // 1.5 × 106 sec-1 at 0.1 mg/ml. The rotation along the major axis as measured on fibrinogen labeled with dansylchloride is DR// = 1.5 x106 sec–1. S is also strongly dependent on concentration, being 7.9 S at 0.1 mg/ml, 8.1 S at 1 mg/ml and 6.6 S at 10 mg/ml.These results fit with an elongated molecule having an axial ratio of 7. They are compatible with a MW of 340’000 only for concentrations above 2 mg/ml, while at lower concentrations (0.1 mg/ml) they agree with a MW of approximately half the accepted value.

scholarly journals Dimensions of short, rodlike macromolecules from translational and rotational diffusion coefficients. Study of the gramicidin dimer

Biopolymers ◽  
1984 ◽  
Vol 23 (4) ◽  
pp. 611-615 ◽  
Author(s):  
Jos� Garc�a De La Torre ◽  
Maria C. Lopez Martinez ◽  
M. Mercedes Tirado
Keyword(s):  
Diffusion Coefficients ◽  
Rotational Diffusion ◽  
Translational And Rotational Diffusion ◽  
Rotational Diffusion Coefficients
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