scholarly journals Deep learning massively accelerates super-resolution localization microscopy

2018 ◽  
Vol 36 (5) ◽  
pp. 460-468 ◽  
Author(s):  
Wei Ouyang ◽  
Andrey Aristov ◽  
Mickaël Lelek ◽  
Xian Hao ◽  
Christophe Zimmer
2020 ◽  
Author(s):  
Anish Mukherjee

The quality of super-resolution images largely depends on the performance of the emitter localization algorithm used to localize point sources. In this article, an overview of the various techniques which are used to localize point sources in single-molecule localization microscopy are discussed and their performances are compared. This overview can help readers to select a localization technique for their application. Also, an overview is presented about the emergence of deep learning methods that are becoming popular in various stages of single-molecule localization microscopy. The state of the art deep learning approaches are compared to the traditional approaches and the trade-offs of selecting an algorithm for localization are discussed.


2018 ◽  
Author(s):  
Marcel Štefko ◽  
Baptiste Ottino ◽  
Kyle M. Douglass ◽  
Suliana Manley

Super-resolution fluorescence microscopy improves spatial resolution, but this comes at a loss of image throughput and presents unique challenges in identifying optimal acquisition parameters. Microscope automation routines can offset these drawbacks, but thus far have required user inputs that presume a priori knowledge about the sample. Here, we develop a flexible illumination control system for localization microscopy comprised of two interacting components that require no sample-specific inputs: a self-tuning controller and a deep learning molecule density estimator that is accurate over an extended range. This system obviates the need to fine-tune parameters and demonstrates the design of modular illumination control for localization microscopy.


Author(s):  
Ruud JG van Sloun ◽  
Oren Solomon ◽  
Matthew Bruce ◽  
Zin Z Khaing ◽  
Hessel Wijkstra ◽  
...  

Author(s):  
Thomas Küstner ◽  
Camila Munoz ◽  
Alina Psenicny ◽  
Aurelien Bustin ◽  
Niccolo Fuin ◽  
...  

2021 ◽  
Vol 52 (S1) ◽  
pp. 187-187
Author(s):  
Yanpeng Cao ◽  
Feng Yu ◽  
Yongming Tang

2021 ◽  
Vol 12 (3) ◽  
pp. 46-47
Author(s):  
Nikita Saxena

Space-borne satellite radiometers measure Sea Surface Temperature (SST), which is pivotal to studies of air-sea interactions and ocean features. Under clear sky conditions, high resolution measurements are obtainable. But under cloudy conditions, data analysis is constrained to the available low resolution measurements. We assess the efficiency of Deep Learning (DL) architectures, particularly Convolutional Neural Networks (CNN) to downscale oceanographic data from low spatial resolution (SR) to high SR. With a focus on SST Fields of Bay of Bengal, this study proves that Very Deep Super Resolution CNN can successfully reconstruct SST observations from 15 km SR to 5km SR, and 5km SR to 1km SR. This outcome calls attention to the significance of DL models explicitly trained for the reconstruction of high SR SST fields by using low SR data. Inference on DL models can act as a substitute to the existing computationally expensive downscaling technique: Dynamical Downsampling. The complete code is available on this Github Repository.


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