scholarly journals Design Principles for Autonomous Illumination Control in Localization Microscopy

2018 ◽  
Author(s):  
Marcel Štefko ◽  
Baptiste Ottino ◽  
Kyle M. Douglass ◽  
Suliana Manley

Super-resolution fluorescence microscopy improves spatial resolution, but this comes at a loss of image throughput and presents unique challenges in identifying optimal acquisition parameters. Microscope automation routines can offset these drawbacks, but thus far have required user inputs that presume a priori knowledge about the sample. Here, we develop a flexible illumination control system for localization microscopy comprised of two interacting components that require no sample-specific inputs: a self-tuning controller and a deep learning molecule density estimator that is accurate over an extended range. This system obviates the need to fine-tune parameters and demonstrates the design of modular illumination control for localization microscopy.

2020 ◽  
Author(s):  
Anish Mukherjee

The quality of super-resolution images largely depends on the performance of the emitter localization algorithm used to localize point sources. In this article, an overview of the various techniques which are used to localize point sources in single-molecule localization microscopy are discussed and their performances are compared. This overview can help readers to select a localization technique for their application. Also, an overview is presented about the emergence of deep learning methods that are becoming popular in various stages of single-molecule localization microscopy. The state of the art deep learning approaches are compared to the traditional approaches and the trade-offs of selecting an algorithm for localization are discussed.


2018 ◽  
Vol 16 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Hongda Wang ◽  
Yair Rivenson ◽  
Yiyin Jin ◽  
Zhensong Wei ◽  
Ronald Gao ◽  
...  

2018 ◽  
Vol 36 (5) ◽  
pp. 460-468 ◽  
Author(s):  
Wei Ouyang ◽  
Andrey Aristov ◽  
Mickaël Lelek ◽  
Xian Hao ◽  
Christophe Zimmer

Author(s):  
Hongda Wang ◽  
Yair Rivenson ◽  
Yiyin Jin ◽  
Zhensong Wei ◽  
Ronald Gao ◽  
...  

Author(s):  
Ruud JG van Sloun ◽  
Oren Solomon ◽  
Matthew Bruce ◽  
Zin Z Khaing ◽  
Hessel Wijkstra ◽  
...  

2020 ◽  
Vol 42 (4) ◽  
pp. 52-56
Author(s):  
Ilijana Vojnovic ◽  
Ulrike Endesfelder

The development of super-resolution microscopy techniques, which are able to achieve resolutions in the nanometre range and as such allow the visualization of subcellular structures and dynamics, has considerably expanded the possibilities of fluorescence microscopy in the life sciences. While a majority of these techniques require highly specialized hardware, single-molecule localization microscopy (SMLM) can be implemented on conventional widefield fluorescence microscopes. Here, we describe what technical upgrades are necessary and discuss some of the difficulties that can be encountered during sample preparation and imaging.


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