Detection of protein–protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

Protein Fragment ◽

Complementation Assay ◽

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.tir117.000385 ◽

2017 ◽

Vol 17 (2) ◽

pp. 373-383 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Extended linkers improve the detection of protein-protein interactions (PPIs) by dihydrofolate reductase protein-fragment complementation assay (DHFR PCA) in living cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.a117.000385 ◽

2018 ◽

Vol 17 (3) ◽

pp. 549-549 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.ra117.000385 ◽

2018 ◽

Vol 17 (2) ◽

pp. 373-383

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Fragment ◽

Complementation Assay ◽

Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

Nature Protocols ◽

10.1038/nprot.2007.356 ◽

2007 ◽

Vol 2 (9) ◽

pp. 2302-2306 ◽

Cited By ~ 21

Author(s):

Ingrid Remy ◽

Galia Ghaddar ◽

Stephen W Michnick

Keyword(s):

Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay

Protein Engineering Protocols ◽

10.1385/1-59745-187-8:249 ◽

2006 ◽

pp. 249-274

Author(s):

François-Xavier Campbell-Valois ◽

Stephen W. Michnick

Keyword(s):

Dihydrofolate Reductase ◽

Binding Domain ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Fragment Complementation Assay ◽

Selection Of

Corrigendum: Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks

10.1101/pdb.corr107813 ◽

2022 ◽

Vol 2022 (1) ◽

pp. pdb.corr107813

Author(s):

Guillaume Diss ◽

Christian R. Landry

Keyword(s):

Dihydrofolate Reductase ◽

Protein Complexes ◽

Interaction Networks ◽

Protein Fragment ◽

Gene Deletions ◽

Complementation Assay ◽

Corrigendum: The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions

10.1101/pdb.corr107812 ◽

2022 ◽

Vol 2022 (1) ◽

pp. pdb.corr107812

Author(s):

Stephen W. Michnick ◽

Emmanuel D. Levy ◽

Christian R. Landry ◽

Jacqueline Kowarzyk ◽

Vincent Messier

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Large Scale ◽

Scale Analysis ◽

Protein Fragment ◽

Large Scale Analysis ◽

Survival Selection ◽

Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives

Applied Microbiology and Biotechnology ◽

10.1007/s00253-008-1624-x ◽

2008 ◽

Vol 80 (4) ◽

pp. 735-743 ◽

Cited By ~ 10

Author(s):

Seiji Shibasaki ◽

Kozue Sakata ◽

Jun Ishii ◽

Akihiko Kondo ◽

Mitsuyoshi Ueda

Keyword(s):

Dihydrofolate Reductase ◽

Protein Fragment ◽

Yeast Protein ◽

Complementation Assay ◽

Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks

10.1101/pdb.prot090035 ◽

2016 ◽

Vol 2016 (11) ◽

pp. pdb.prot090035 ◽

Cited By ~ 1

Author(s):

Guillaume Diss ◽

Christian R. Landry

Keyword(s):

Dihydrofolate Reductase ◽

Protein Complexes ◽

Interaction Networks ◽

Protein Fragment ◽

Gene Deletions ◽

Complementation Assay ◽

The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions

10.1101/pdb.prot090027 ◽

2016 ◽

Vol 2016 (11) ◽

pp. pdb.prot090027 ◽

Cited By ~ 4

Author(s):

Stephen W. Michnick ◽

Emmanuel D. Levy ◽

Christian R. Landry ◽

Jacqueline Kowarzyk ◽

Vincent Messier

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Large Scale ◽

Scale Analysis ◽

Protein Fragment ◽

Large Scale Analysis ◽

Survival Selection ◽