Impregnation and deposition-precipitation (DP) methods were employed for the preparation of Au nanoparticles supported on SBA-15, and the influences of various reductants were systematically surveyed. The dispersion and particle size of gold loaded on SBA-15 were determined by XRD, SEM and TEM. It has been found that reductants have effect on the size and dispersion of Au NPs, resulting in different catalytic properties for aerobic oxidation of glucose. The Au NPs on SBA-15 using KBH4as reductant was approximately 5-6 nm, which exhibited excellent catalytic activity.