scholarly journals Flow cytometric microsphere-based immunoassay as a novel non-radiometric method for the detection of glutamic acid decarboxylase autoantibodies in type 1 diabetes mellitus

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3017 ◽  
Author(s):  
Luciano L. Guerra ◽  
Aldana Trabucchi ◽  
Natalia I. Faccinetti ◽  
Ruben F. Iacono ◽  
Daniela B. Ureta ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Radiometric Method ◽  
Acid Decarboxylase ◽  
Flow Cytometric ◽  
Glutamic Acid Decarboxylase Autoantibodies

Peptide-specific cytotoxicity of T lymphocytes against glutamic acid decarboxylase and insulin in type 1 diabetes mellitus

2001 ◽  
Vol 51 (3) ◽  
pp. 173-179 ◽  
Author(s):  
Kayo Kimura ◽  
Tomoyuki Kawamura ◽  
Shinji Kadotani ◽  
Hiroshi Inada ◽  
Shizuhiro Niihira ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
T Lymphocytes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Specific Cytotoxicity

scholarly journals Time-resolved Fluorometric Assay for Detection of Autoantibodies to Glutamic Acid Decarboxylase (GAD65)

2003 ◽  
Vol 49 (6) ◽  
pp. 908-915 ◽  
Author(s):  
Matti Ankelo ◽  
Annette Westerlund-Karlsson ◽  
Jorma Ilonen ◽  
Mikael Knip ◽  
Kaisa Savola ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Serum Samples ◽  
Analytical Range ◽  
Radiobinding Assay

Abstract Background: Type 1 diabetes mellitus results from destruction of the pancreatic insulin-producing beta cells by a chronic autoimmune process. Methods are needed for the detection of circulating autoantibodies to glutamic acid decarboxylase (GAD65), a major marker of this process. Methods: Streptavidin-coated microtiter plates were incubated with biotinylated GAD65, and after incubation with serum samples from patients with type 1 diabetes mellitus and control individuals, europium-labeled GAD65 was added. After washing steps, the delayed fluorescence was measured in duplicate in a fluorometer. Samples collected from 100 patients with newly diagnosed type 1 diabetes mellitus and 100 healthy controls were measured by the new assay and by a radiobinding assay. Results: The detection limit of the new assay was 1.49 WHO units/mL, the calibration curve was linear to 4 140 WHO units/mL, and no hook effect was observed up to 41 400 WHO units/mL. The intraassay CV was 2.1–6.3% over the calibration range. For patient serum samples, the intraassay, interassay, and total CVs were 5.4–7.0%, 9.8–13%, and 12–14%, respectively. Compared with conventional radioimmunologic methods, the analytical range was broader and the analysis time required to perform the measurements was shorter. At a cutoff with 99% specificity, the new assay and the radiobinding assay were positive in 71 and 67 patients, respectively. Conclusions: The new assay provides a rapid and sensitive nonradioactive method applicable for large-scale screening for beta-cell autoimmunity. It has a broad linear analytical range, is easy to perform and automate, and has sensitivity and specificity comparable to those for the conventional radioisotope assay.

Immune reactivity to glutamic acid decarboxylase 65 in stiff-man syndrome and type 1 diabetes mellitus

The Lancet ◽  
2000 ◽  
Vol 356 (9223) ◽  
pp. 31-35 ◽  
Author(s):  
Tobias Lohmann ◽  
Mohammed Hawa ◽  
R David G Leslie ◽  
Russell Lane ◽  
Jean Picard ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Immune Reactivity ◽  
Stiff Man Syndrome ◽  
Glutamic Acid Decarboxylase 65

Enterovirus-related type 1 diabetes mellitus and antibodies to glutamic acid decarboxylase in Japan

2004 ◽  
Vol 49 (2) ◽  
pp. 147-151 ◽  
Author(s):  
H KAWASHIMA ◽  
T IHARA ◽  
H IOI ◽  
S OANA ◽  
S SATO ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Glutamic Acid ◽  
Type 1 Diabetes Mellitus ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase
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