An electrochemical sensor based on DNA polymerase and HRP-SiO2 nanoparticles for the ultrasensitive detection of K-ras gene point mutation

RSC Advances ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 8669-8676 ◽  
Author(s):  
Chengfei Zhao ◽  
Feng Gao ◽  
Shaohuang Weng ◽  
Qicai Liu ◽  
Liqing Lin ◽  
...  
Keyword(s):  
Point Mutation ◽  
Dna Polymerase ◽  
Sio2 Nanoparticles ◽  
Ultrasensitive Detection ◽  
Dna Sensor ◽  
Dna Polymerase I ◽  
Ras Gene ◽  
Target Dna ◽  
Polymerase I ◽  
Mixture Sample

We developed a DNA sensor for the precise detection of point mutation of K-ras gene. The sensor was based on DNA replication, which employed the DNA polymerase I and the principle of base pairing, and can detect target DNA in mixture sample.

scholarly journals Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Ani Saghatelyan ◽  
Hovik Panosyan ◽  
Armen Trchounian ◽  
Nils‐Kåre Birkeland
Keyword(s):  
Dna Polymerase ◽  
Extreme Thermophile ◽  
Dna Polymerase I ◽  
Polymerase I

Site-specific modification of E. coli DNA polymerase I large fragment with pyridoxal 5'-phosphate

Biochemistry ◽  
1984 ◽  
Vol 23 (9) ◽  
pp. 2073-2078 ◽  
Author(s):  
Anup K. Hazra ◽  
Sevilla Detera-Wadleigh ◽  
Samuel H. Wilson
Keyword(s):  
Dna Polymerase ◽  
Large Fragment ◽  
Dna Polymerase I ◽  
Site Specific ◽  
E Coli ◽  
Specific Modification ◽  
Polymerase I

scholarly journals Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Johannes Hohlbein ◽  
Louise Aigrain ◽  
Timothy D. Craggs ◽  
Oya Bermek ◽  
Olga Potapova ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Nucleotide Insertion ◽  
Polymerase I
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