scholarly journals A simple check valve for microfluidic point of care diagnostics

Lab on a Chip ◽  
2016 ◽  
Vol 16 (22) ◽  
pp. 4436-4444 ◽  
Author(s):  
C. S. Ball ◽  
R. F. Renzi ◽  
A. Priye ◽  
R. J. Meagher
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Check Valve ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Testing ◽  
Point Of Care Diagnostics

Laser cut microfluidic check valves enable staged reagent delivery, pumping, and point of care nucleic acid amplification testing.

Paper-based nucleic acid amplification tests for point-of-care diagnostics

The Analyst ◽  
2018 ◽  
Vol 143 (10) ◽  
pp. 2213-2234 ◽  
Author(s):  
Navjot Kaur ◽  
Bhushan J. Toley
Keyword(s):  
Nucleic Acid ◽  
Critical Review ◽  
Point Of Care ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests ◽  
Point Of Care Diagnostics

A critical review of paper-based nucleic acid amplification tests with a focus on integration and sequence of operations.

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

scholarly journals Accuracy of antigen and nucleic acid amplification testing on saliva and naopharyngeal samples for detection of SARS-CoV-2 in ambulatory care

2021 ◽  
Author(s):  
Solen Kerneis ◽  
Caroline Elie ◽  
Jacques Fourgeaud ◽  
Laure Choupeaux ◽  
Severine Mercier Delarue ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Diagnostic Accuracy ◽  
Point Of Care ◽  
Saliva Sample ◽  
Nucleic Acid Amplification ◽  
Ambulatory Setting ◽  
Current Standard ◽  
Funding Sources ◽  
Nucleic Acid Amplification Testing ◽  
Prospective Participant

Background: Nasopharyngeal sampling for nucleic acid amplification testing (NAAT) is the current standard diagnostic test for of coronavirus disease 2019 (COVID-19). However, the NAAT technique is lengthy and nasopharyngeal sampling requires trained personnel. Saliva NAAT represents an interesting alternative but diagnostic performances vary widely between studies. Objective: To assess the diagnostic accuracy of a nasopharyngeal point-of-care antigen (Ag) test and of saliva NAAT for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), as compared to nasopharyngeal NAAT. Design: Prospective participant enrollment from 19 October through 18 December 2020. Setting: Two community COVID-19 screening centers in Paris, France. Participants: 1452 ambulatory children and adults referred for SARS-CoV-2 testing. Interventions: NAAT on a saliva sample (performed with three different protocols for pre-processing, amplification and detection of SARS-CoV-2) and Ag testing on a nasopharyngeal sample. Measurements: Performance of saliva NAAT and nasopharyngeal Ag testing. Results: Overall, 129/1443 (9%) participants tested positive on nasopharyngeal NAAT (102/564 [18%] in symptomatic and 27/879 [3%] in asymptomatic participants). Sensitivity was of 94% (95% CI, 86% to 98%), 23% (CI, 14% to 35%), 94% (CI, 88% to 97%) and 96% (CI, 91% to 99%) for the nasopharyngeal Ag test and the three different protocols of saliva NAAT, respectively. Estimates of specificity were above 95% for all methods. Diagnostic accuracy was similar in symptomatic and asymptomatic individuals. Limitations: Few children (n=122, 8%) were included. Conclusion: In the ambulatory setting, diagnostic accuracy of nasopharyngeal Ag testing and of saliva NAAT seems similar to that of nasopharyngeal NAAT, subject to strict compliance with specific pre-processing and amplification protocols. Registration number: NCT04578509 Funding Sources: French Ministry of Health and the Assistance Publique-Hopitaux de Paris Foundation.

scholarly journals Combined SARS-CoV-2 nucleic acid amplification testing and respiratory virus panel RT-PCR on the Hologic Panther Fusion system

2021 ◽  
Vol 138 ◽  
pp. 104792
Author(s):  
Bryan A. Stevens ◽  
Catherine A. Hogan ◽  
Kenji O. Mfuh ◽  
Ghazala Khan ◽  
Malaya K. Sahoo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Respiratory Virus ◽  
Nucleic Acid Amplification ◽  
Fusion System ◽  
Rt Pcr ◽  
Nucleic Acid Amplification Testing
Sign in / Sign up

Export Citation Format

Share Document