Planar hydrodynamic traps and buried channels for bead and cell trapping and releasing

Lab on a Chip ◽

10.1039/d1lc00463h ◽

2021 ◽

Author(s):

Clémentine Lipp ◽

Kevin Uning ◽

Jonathan Cottet ◽

Daniel Migliozzi ◽

Arnaud Bertsch ◽

...

Keyword(s):

Microfluidic Channels ◽

Cell Trapping ◽

New Process ◽

Buried Channels

A new process for the fabrication of two superposed layers of microfluidic channels connected by vias is used to trap and release particles in a transparent chip. Parallel manipulation of beads is studied and the rules for cell trapping are defined.

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Trapping Single Cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip

Materials Science Forum ◽

10.4028/www.scientific.net/msf.700.188 ◽

2011 ◽

Vol 700 ◽

pp. 188-194 ◽

Cited By ~ 1

Author(s):

Siti Noorjannah Ibrahim ◽

Lynn Murray ◽

John J. Evans ◽

Maan M. Alkaisi

Keyword(s):

Single Cells ◽

Contact Layer ◽

Trapping Efficiency ◽

Microfluidic Channels ◽

Back Contact ◽

Insulation Layer ◽

Ac Electrokinetics ◽

Cell Trapping ◽

Nickel Chromium ◽

Single Cell Trapping

AC electrokinetics is one of many methods used to move particles in microfluidic channels. This paper presents single cell trapping efficacy using dielectrophoresis (DEP) force of two biochip designs; a planar biochip and the new sandwiched-insulation with back contact (SIBC) biochip. The new biochip, is structured on a glass slide, consists of microelectrode arrays patterned on top of Nickel-Chromium (NiCr) and Gold (Au) layers. Prior to the microelectrode patterning, a back contact layer of NiCr and Au was coated with SU-8 2005. Then, the SU-8 2005 or the insulation layer was patterned with arrays of microcavities. In contrast, the planar biochip consists of 2 layers; an SU-8 2005 insulation layer and NiCr and Au metal layers constructed on a Silicon Nitride (Si3N4) substrate. The electric field intensity results simulated using Comsol v3.5a software indicated that DEP force generated from the SIBC biochip are greater than the planar biochip design. Results from experiment with polystyrene microbeads and Ishikawa cancer cells also showed that the SIBC biochip has higher trapping efficiency than the planar biochip. These promising results indicate that the SIBC biochip is capable of trapping single cells and can be used to facilitate studies on intracellular activities using surface the replicating technique known as the Bioimprint technique.

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Hydrodynamic lift for single cell manipulation in a femtosecond laser fabricated optofluidic chip

Optofluidics Microfluidics and Nanofluidics ◽

10.1515/optof-2017-0003 ◽

2017 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

Francesca Bragheri ◽

Roberto Osellame

Keyword(s):

Femtosecond Laser ◽

Single Cell ◽

Optical Waveguides ◽

Cell Manipulation ◽

Microfluidic Channels ◽

Hydrodynamic Focusing ◽

Cell Trapping ◽

Femtosecond Laser Micromachining ◽

Single Cell Trapping ◽

Optofluidic Chip

AbstractSingle cell sorting based either on fluorescence or on mechanical properties has been exploited in the last years in microfluidic devices. Hydrodynamic focusing allows increasing the efficiency of theses devices by improving the matching between the region of optical analysis and that of cell flow. Here we present a very simple solution fabricated by femtosecond laser micromachining that exploits flow laminarity in microfluidic channels to easily lift the sample flowing position to the channel portion illuminated by the optical waveguides used for single cell trapping and analysis.

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