Hydrodynamic lift for single cell manipulation in a femtosecond laser fabricated optofluidic chip

AbstractSingle cell sorting based either on fluorescence or on mechanical properties has been exploited in the last years in microfluidic devices. Hydrodynamic focusing allows increasing the efficiency of theses devices by improving the matching between the region of optical analysis and that of cell flow. Here we present a very simple solution fabricated by femtosecond laser micromachining that exploits flow laminarity in microfluidic channels to easily lift the sample flowing position to the channel portion illuminated by the optical waveguides used for single cell trapping and analysis.

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Single cell trapping and stretching in an optofluidic chip fabricated by femtosecond laser micromachining

2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference (CLEO EUROPE/EQEC) ◽

10.1109/cleoe.2011.5942936 ◽

2011 ◽

Cited By ~ 1

Author(s):

F. Bragheri ◽

L. Ferrara ◽

P. Minzioni ◽

I. Cristiani ◽

K. C. Vishnubhatla ◽

...

Keyword(s):

Femtosecond Laser ◽

Single Cell ◽

Laser Micromachining ◽

Cell Trapping ◽

Femtosecond Laser Micromachining ◽

Single Cell Trapping ◽

Optofluidic Chip

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Single cell trapping and stretching in a femtosecond laser fabricated optofluidic chip

2011 International Workshop on Biophotonics ◽

10.1109/iwbp.2011.5954861 ◽

2011 ◽

Author(s):

L. Ferrara ◽

F. Bragheri ◽

P. Minzioni ◽

I. Cristiani ◽

K. C. Vishnubhatla ◽

...

Keyword(s):

Femtosecond Laser ◽

Single Cell ◽

Cell Trapping ◽

Single Cell Trapping ◽

Optofluidic Chip

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Optofluidic chip for single cell trapping and stretching fabricated by a femtosecond laser

Journal of Biophotonics ◽

10.1002/jbio.201000011 ◽

2010 ◽

Vol 3 (4) ◽

pp. 234-243 ◽

Cited By ~ 49

Author(s):

Francesca Bragheri ◽

Lorenzo Ferrara ◽

Nicola Bellini ◽

Krishna C. Vishnubhatla ◽

Paolo Minzioni ◽

...

Keyword(s):

Femtosecond Laser ◽

Single Cell ◽

Cell Trapping ◽

Single Cell Trapping ◽

Optofluidic Chip

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Simulation of Single Cell Trapping Via Hydrodynamic Manipulation

Jurnal Teknologi ◽

10.11113/jt.v69.3306 ◽

2014 ◽

Vol 69 (8) ◽

Author(s):

Amelia Ahmad Khalili ◽

Mohd Ariffanan Mohd Basri ◽

Mohd Ridzuan Ahmad

Keyword(s):

Yeast Cell ◽

Single Cell ◽

Flow Rate ◽

Cell Model ◽

Cell Manipulation ◽

Hydrodynamic Resistance ◽

Cell Trapping ◽

Trapping Model ◽

Single Cell Trapping ◽

Suction Flow

Microfluidic devices are important for the single cell analysis such as cell mechanical and electrical characterization. Single cell characterization could be related to many significant applications including early disease diagnosis. However to perform the single cell manipulation, firstly a single cell have to be isolated and a platform for the cell manipulation have to be provided. One of the methods to trap a single cell is by using hydrodynamic trapping in the microfluidic channel. This study provides a finite element model for single cell trapping for a yeast cell model. The objectives of the simulations are to obtain the appropriate channels’ geometry and optimized ratio of the fluid’s inlet and suction flow rate to trap a single yeast cell. Trap channel was designed to trap a 5μm yeast cell with a suction hole placed in the end of the trap channel. Design geometry and the ratio of fluid flow rates for the cell trapping model were studied using the hydrodynamic resistance concept. The analysis was carried out using numerical solutions from the finite element ABAQUS-FEA software. Using the cell trapping model, a single yeast cell able to be trapped into the trap channel with optimized channel’s suction hole’s geometry and appropriate fluid’s inlet and suction flow rate ratio. The appropriate QTrap/QMain ratio to perform cell trapping using hydrodynamic resistance concept is the ratio value above 1. A 5 μm yeast cell model able to be trap inside a trap channel with the height, width and length of 7 μm by manipulating the suction hole’s flow rate of 1.5 and 2.0 μm of height, 7 and 3 μm of length and width, respectively which situated at the centre edge of the trap channel.

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A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽

10.1039/d1lc00302j ◽

2021 ◽

Author(s):

Huichao Chai ◽

Yongxiang Feng ◽

Fei Liang ◽

Wenhui Wang

Keyword(s):

Chemical Analysis ◽

Single Cell ◽

Microfluidic Device ◽

Single Cells ◽

Cell Isolation ◽

Cell Trapping ◽

Analysis Techniques ◽

Single Cell Trapping ◽

Single Cell Isolation ◽

Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

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Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

The Scientific World JOURNAL ◽

10.1155/2014/929163 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

B. Deng ◽

X. F. Li ◽

D. Y. Chen ◽

L. D. You ◽

J. B. Wang ◽

...

Keyword(s):

Single Cell ◽

Microfluidic Device ◽

Single Cell Analysis ◽

Fine Tuning ◽

Trapping Efficiency ◽

Cell Analysis ◽

Cell Trapping ◽

Micro Channels ◽

Single Cell Trapping ◽

Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

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