Interallelic complementation provides genetic evidence for the multimeric organization of the Phycomyces blakesleeanus phytoene dehydrogenase

2002 ◽  
Vol 269 (3) ◽  
pp. 902-908 ◽  
Author(s):  
Catalina Sanz ◽  
María I. Alvarez ◽  
Margarita Orejas ◽  
Antonio Velayos ◽  
Arturo P. Eslava ◽  
...  
Keyword(s):  
Genetic Evidence ◽  
Phycomyces Blakesleeanus ◽  
Phytoene Dehydrogenase ◽  
Interallelic Complementation

Inhibition of Carotene Biosynthesis in Cell Extracts of Phycomyces blakesleeanus

1984 ◽  
Vol 39 (5) ◽  
pp. 460-463 ◽  
Author(s):  
Peter M. Bramley ◽  
Ian E. Clarke ◽  
Gerhard Sandmann ◽  
Peter Böger
Keyword(s):  
Competitive Inhibition ◽  
Enzymic Activity ◽  
Phycomyces Blakesleeanus ◽  
Cell Extracts ◽  
Phytoene Dehydrogenase ◽  
Β Carotene

Cell extracts of the C115 (β-carotene-accumulating) strain of Phycomyces blakesleeanus were incubated with either [2-14C]MVA or [1-14C]IPP and a range of possible inhibitors of carotenogenesis, including bleaching herbicides, biphenyl compounds and geranylacetone. Several of these compounds were potent inhibitors of β-carotene formation and caused the accumulation of phytoene. No other carotenes were found to accumulate, in vitro.The structures of these inhibitors, compared to that of phytoene, suggest that they affect the enzymic activity of “phytoene dehydrogenase”, possibly by competitive inhibition.

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