Serum IgA and IgG functional antibodies and their subclasses to Streptococcus pneumoniae capsular antigen found in two aged-matched cohorts of children with and without otitis media with effusion

2003 ◽  
Vol 28 (4) ◽  
pp. 335-340 ◽  
Author(s):  
A.B. Drake-Lee ◽  
R.G. Hughes ◽  
C. Dunn
Keyword(s):  
Streptococcus Pneumoniae ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Capsular Antigen ◽  
Serum Iga ◽  
Functional Antibodies

Bacteriology of chronic otitis media with effusion

1989 ◽  
Vol 103 (4) ◽  
pp. 369-371 ◽  
Author(s):  
C. Diamond ◽  
P. R. Sisson ◽  
A. M. Kearns ◽  
H. R. Ingham
Keyword(s):  
Streptococcus Pneumoniae ◽  
Haemophilus Influenzae ◽  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Anaerobic Bacteria ◽  
Chronic Otitis Media

AbstractSamples of middle ear effusions from 102 children with serous and mucoid otitis media were cultured for mycoplasmas and bacteria. No sample yielded mycoplasmas but bacteria were cultured from 48 (47 per cent). Organisms commonly regarded as pathogens were present in 25 samples (Haemophilus influenzae 17, Streptococcus pneumoniae four, other streptococci four). The only sample from which anaerobic bacteria were isolated was from a patient with cholesteatoma.

scholarly journals Immunization with Native or Recombinant Streptococcus pneumoniae Neuraminidase Affords Protection in the Chinchilla Otitis Media Model

2004 ◽  
Vol 72 (7) ◽  
pp. 4309-4313 ◽  
Author(s):  
J. P. Long ◽  
H. H. Tong ◽  
T. F. DeMaria
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae ◽  
Otitis Media ◽  
Virulence Factor ◽  
Otitis Media With Effusion ◽  
Nasopharyngeal Colonization ◽  
First Time

ABSTRACT Streptococcus pneumoniae neuraminidase has been implicated as a virulence factor in the pathogenesis of pneumococcal otitis media. In this study, native neuraminidase was partially purified from cultures of S. pneumoniae by serial chromatography with DEAE-Sepharose and Sephacryl S-200. Recombinant neuraminidase, a 3,038-bp fragment of the neuraminidase A (nanA) gene, was cloned into the pET-28b vector and then expressed at high levels in Escherichia coli. Chinchillas were immunized subcutaneously with either the gel-purified native or recombinant neuraminidase, and all responded with elevated titers of antineuraminidase antibody in serum. Immunization with neuraminidase resulted in a significant reduction in nasopharyngeal colonization as well as in the incidence of otitis media with effusion. These data demonstrate for the first time that neuraminidase affords protection against S. pneumoniae nasopharyngeal colonization and experimental otitis media.

PCR Assay for Detecting Streptococcus pneumoniae in the Middle Ear of Children with Otitis Media with Effusion

1996 ◽  
Vol 116 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Jussi Jero ◽  
Anni Virolainen ◽  
Paula Salo ◽  
Maija Leinonen ◽  
Juhani Eskola ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Pcr Assay
Sign in / Sign up

Export Citation Format

Share Document