Molecular characterization of a peroxiredoxin from the hard tick Haemaphysalis longicornis

2001 ◽  
Vol 10 (2) ◽  
pp. 121-129 ◽  
Author(s):  
N. Tsuji ◽  
T. Kamio ◽  
T. Isobe ◽  
K. Fujisaki
Keyword(s):  
Molecular Characterization ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

Cloning and molecular characterization of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis

2004 ◽  
Vol 34 (8) ◽  
pp. 799-808 ◽  
Author(s):  
Takeharu Miyoshi ◽  
Naotoshi Tsuji ◽  
M Khyrul Islam ◽  
Tsugihiko Kamio ◽  
Kozo Fujisaki
Keyword(s):  
Molecular Characterization ◽  
Serine Proteinase ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

scholarly journals EB8 Molecular characterization of a peroxiredoxin from the hard tick Haemaphysalis longicornis

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 141
Author(s):  
Naotoshi TSUJI ◽  
Myung Jo YOO ◽  
Harue KASUGA ◽  
Takashi ISOBE ◽  
Tsugihiko KAMIO ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis

2001 ◽  
Vol 32 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Myungjo You ◽  
Xuenan Xuan ◽  
Naotoshi Tsuji ◽  
Tsugihiko Kamio ◽  
Ikuo Igarashi ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Troponin I ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis

2006 ◽  
Vol 36 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Damdinsuren Boldbaatar ◽  
Chummy Sikalizyo Sikasunge ◽  
Badgar Battsetseg ◽  
Xuenan Xuan ◽  
Kozo Fujisaki
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
Aspartic Protease ◽  
Hard Tick ◽  
Haemaphysalis Longicornis

scholarly journals Enzymatic Characterization of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis

2004 ◽  
Vol 66 (10) ◽  
pp. 1195-1198 ◽  
Author(s):  
Takeharu MIYOSHI ◽  
Naotoshi TSUJI ◽  
M. Khyrul ISLAM ◽  
Tsugihiko KAMIO ◽  
Kozo FUJISAKI
Keyword(s):  
Serine Proteinase ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Enzymatic Characterization

Valosin-containing protein from the hard tick Haemaphysalis longicornis: effects of dsRNA-mediated HlVCP gene silencing

2007 ◽  
Vol 85 (3) ◽  
pp. 384-394 ◽  
Author(s):  
Damdinsuren Boldbaatar ◽  
Badgar Battsetseg ◽  
Takeshi Hatta ◽  
Takeharu Miyoshi ◽  
Naotoshi Tsuji ◽  
...  
Keyword(s):  
Blood Feeding ◽  
Pcr Analysis ◽  
Hard Tick ◽  
Haemaphysalis Longicornis ◽  
Native Protein ◽  
Rt Pcr ◽  
Future Studies ◽  
Atpase Gene ◽  
A Domains

We report the cloning and characterization of a cDNA encoding the valosin-containing protein (VCP) from the Haemaphysalis longicornis tick (HlVCP). The full-length HlVCP is 2782 bp and codes for 808 amino acids of a deduced protein with a predicted molecular mass of 89.9 kDa. The domain structure analysis revealed that the deduced protein has 2 Walker A domains, 2 Walker B domains, a Cdc48 domain, and a polyQ-binding domain. The mouse anti-HlVCP serum recognized a 97 kDa native protein in the salivary glands, midgut, and synganglion. RT–PCR analysis revealed that the native VCP was expressed throughout the developing stages and in tick organs. HlVCP silencing resulted in a decrease in tick body mass after blood feeding. This study not only contributes to a growing understanding of the ATPase gene family but also lays the groundwork for future studies on protein secretion and host–tick interaction. This study is the first report of the VCP gene from Chelicerata, which include spiders, scorpions, and ticks.

scholarly journals Functional characterization of two defensins, HlDFS1 and HlDFS2, from the hard tick Haemaphysalis longicornis

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Ta Sun ◽  
Wen Pan ◽  
Yanhui Song ◽  
Jingpin Zhang ◽  
Jingwen Wang ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Hard Tick ◽  
Haemaphysalis Longicornis
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