A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants

Paraskevi Tavladoraki; Alessandra Girotti; Marcello Donini; Francisco Javier Arias; Camillo Mancini; Veronica Morea; Roberta Chiaraluce; Valerio Consalvi; Eugenio Benvenuto

doi:10.1046/j.1432-1327.1999.00443.x

A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants

European Journal of Biochemistry ◽

10.1046/j.1432-1327.1999.00443.x ◽

1999 ◽

Vol 262 (2) ◽

pp. 617-624 ◽

Cited By ~ 41

Author(s):

Paraskevi Tavladoraki ◽

Alessandra Girotti ◽

Marcello Donini ◽

Francisco Javier Arias ◽

Camillo Mancini ◽

...

Keyword(s):

Escherichia Coli ◽

Transgenic Plants ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Production of a biotinylated single-chain antibody fragment in the cytoplasm of Escherichia coli

Journal of Immunological Methods ◽

10.1016/j.jim.2003.10.008 ◽

2004 ◽

Vol 284 (1-2) ◽

pp. 165-175 ◽

Cited By ~ 30

Author(s):

Ville Santala ◽

Urpo Lamminmäki

Keyword(s):

Escherichia Coli ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Purification and on-column refolding of a single-chain antibody fragment against rabies virus glycoprotein expressed in Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2016.05.004 ◽

2016 ◽

Vol 126 ◽

pp. 26-32 ◽

Cited By ~ 4

Author(s):

Hualong Xi ◽

Ruosen Yuan ◽

Xiaoxu Chen ◽

Tiejun Gu ◽

Yue Cheng ◽

...

Keyword(s):

Escherichia Coli ◽

Rabies Virus ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Rabies Virus Glycoprotein ◽

Virus Glycoprotein ◽

Single Chain Antibody Fragment

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Improved biological activity of a single chain antibody fragment against human epidermal growth factor receptor 2 (HER2) expressed in the periplasm of Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2015.07.005 ◽

2015 ◽

Vol 116 ◽

pp. 66-74 ◽

Cited By ~ 4

Author(s):

Vajihe Akbari ◽

Hamid Mir Mohammad Sadeghi ◽

Abbas Jafarian-Dehkordi ◽

Daryoush Abedi ◽

C. Perry Chou

Keyword(s):

Escherichia Coli ◽

Epidermal Growth Factor Receptor ◽

Biological Activity ◽

Growth Factor ◽

Growth Factor Receptor ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Epidermal Growth ◽

Single Chain Antibody Fragment

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Bacterial aspects associated with the expression of a single-chain antibody fragment in Escherichia coli

Applied Microbiology and Biotechnology ◽

10.1007/s002530050299 ◽

1994 ◽

Vol 42 (4) ◽

pp. 595-603 ◽

Cited By ~ 4

Author(s):

J. E. Somerville ◽

S. C. Goshorn ◽

H. P. Fell ◽

R. P. Darveau

Keyword(s):

Escherichia Coli ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression

Applied Microbiology and Biotechnology ◽

10.1007/s00253-002-1190-6 ◽

2003 ◽

Vol 61 (2) ◽

pp. 123-132 ◽

Cited By ~ 13

Author(s):

P. Oelschlaeger ◽

S. Lange ◽

J. Schmitt ◽

M. Siemann ◽

M. Reuss ◽

...

Keyword(s):

Escherichia Coli ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

In Vitro Expression ◽

Single Chain Antibody Fragment

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Functional expression of a single-chain antibody fragment against human epidermal growth factor receptor 2 (HER2) in Escherichia coli

Journal of Industrial Microbiology & Biotechnology ◽

10.1007/s10295-014-1437-0 ◽

2014 ◽

Vol 41 (6) ◽

pp. 947-956 ◽

Cited By ~ 13

Author(s):

Vajihe Akbari ◽

Hamid Mir Mohammad Sadeghi ◽

Abbas Jafrian-Dehkordi ◽

Daryoush Abedi ◽

C. Perry Chou

Keyword(s):

Escherichia Coli ◽

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Growth Factor Receptor ◽

Antibody Fragment ◽

Functional Expression ◽

Single Chain ◽

Single Chain Antibody ◽

Epidermal Growth ◽

Single Chain Antibody Fragment

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Expression in Escherichia coli of soluble and M13 phage-displayed forms of a single-chain antibody fragment specific for digoxin: assessment in a novel drug immunoassay

Immunotechnology ◽

10.1016/1380-2933(95)00004-6 ◽

1995 ◽

Vol 1 (1) ◽

pp. 41-52 ◽

Cited By ~ 9

Author(s):

Isabelle Navarro-Teulon ◽

Sylvie Peraldi-Roux ◽

Thierry Bernardi ◽

Mariana Marin ◽

Marc Piechaczyk ◽

...

Keyword(s):

Escherichia Coli ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Expression In Escherichia Coli ◽

M13 Phage ◽

Single Chain Antibody Fragment ◽

Novel Drug

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Bacterial aspects associated with the expression of a single-chain antibody fragment in Escherichia coli

Applied Microbiology and Biotechnology ◽

10.1007/bf00173926 ◽

1994 ◽

Vol 42 (4) ◽

pp. 595-603 ◽

Cited By ~ 14

Author(s):

J. E. Somerville ◽

S. C. Goshorn ◽

H. P. Fell ◽

R. P. Darveau

Keyword(s):

Escherichia Coli ◽

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Single Chain Antibody Fragment

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Brolucizumab: a novel anti-VEGF humanized single-chain antibody fragment for treating w-AMD

Expert Opinion on Biological Therapy ◽

10.1080/14712598.2021.1915278 ◽

2021 ◽

Vol 21 (5) ◽

pp. 553-561

Author(s):

Lorenzo Ferro Desideri ◽

Carlo Enrico Traverso ◽

Massimo Nicolò

Keyword(s):

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Anti Vegf ◽

Single Chain Antibody Fragment

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423. Isolation of an Adeno-Associated Virus (AAV)-Specific Camelid-Derived Single Chain Antibody Fragment: A Novel Tool for Purification of AAV Vectors of Different Serotypes

Molecular Therapy ◽

10.1016/j.ymthe.2004.06.344 ◽

2004 ◽

Vol 9 ◽

pp. S162 ◽

Cited By ~ 2

Keyword(s):

Antibody Fragment ◽

Single Chain ◽

Single Chain Antibody ◽

Adeno Associated Virus ◽

Single Chain Antibody Fragment

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