scholarly journals Combination of pulsed-field gel electrophoresis (PFGE) and single-enzyme amplified fragment length polymorphism (SAFLP) for differentiation of multiresistant Salmonella enterica serotype typhimurium

2002 ◽  
Vol 8 (3) ◽  
pp. 154-161 ◽  
Author(s):  
S. Sood ◽  
T. Peters ◽  
L.R. Ward ◽  
E.J. Threlfall
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Single Enzyme

scholarly journals Fluorescent Amplified-Fragment Length Polymorphism Genotyping of Salmonella enterica subsp. enterica Serovars and Comparison with Pulsed-Field Gel Electrophoresis Typing

2000 ◽  
Vol 38 (4) ◽  
pp. 1623-1627 ◽  
Author(s):  
Bjørn-Arne Lindstedt ◽  
Even Heir ◽  
Traute Vardund ◽  
Georg Kapperud
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Automated Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. entericacomprising seven different serovars using the restriction enzymesEcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprinting S. enterica subsp. entericaserovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.

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