scholarly journals In vitro synthesis of full-length DNA transcripts of Rous sarcoma virus RNA by viral DNA polymerase.

1975 ◽  
Vol 72 (12) ◽  
pp. 4895-4899 ◽  
Author(s):  
R. P. Junghans ◽  
P. H. Duesberg ◽  
C. A. Knight
Keyword(s):  
Dna Polymerase ◽  
Rous Sarcoma Virus ◽  
Full Length ◽  
Viral Dna ◽  
In Vitro Synthesis ◽  
Rous Sarcoma ◽  
Virus Rna ◽  
Sarcoma Virus

THE INITIATION SITES OF ROUS SARCOMA VIRUS RNA-DIRECTED DNA SYNTHESIS IN VITRO

1976 ◽  
pp. 147-159
Author(s):  
Linda M. Cashion ◽  
R.H. Joho ◽  
Margaret A. Planitz ◽  
M.A. Billeter ◽  
C. Weissmann
Keyword(s):  
Dna Synthesis ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Virus Rna ◽  
Sarcoma Virus

scholarly journals Rous Sarcoma Virus (RSV) Integration In Vivo: a CA Dinucleotide Is Not Required in U3, and RSV Linear DNA Does Not Autointegrate

2007 ◽  
Vol 82 (1) ◽  
pp. 503-512 ◽  
Author(s):  
Jangsuk Oh ◽  
Kevin W. Chang ◽  
Rafal Wierzchoslawski ◽  
W. Gregory Alvord ◽  
Stephen H. Hughes
Keyword(s):  
Rous Sarcoma Virus ◽  
In Vitro Assays ◽  
Viral Dna ◽  
Experimental Conditions ◽  
Linear Dna ◽  
Rous Sarcoma ◽  
Viral Dnas ◽  
Sarcoma Virus

ABSTRACT The sequences required for integration of retroviral DNA have been analyzed in vitro. However, the in vitro experiments do not agree on which sequences are required for integration: for example, whether or not the conserved CA dinucleotide in the 3′ end of the viral DNA is required for normal integration. At least a portion of the problem is due to differences in the experimental conditions used in the in vitro assays. To avoid the issue of what experimental conditions to use, we took an in vivo approach. We made mutations in the 5′ end of the U3 sequence of the Rous sarcoma virus (RSV)-derived vector RSVP(A)Z. We present evidence that, in RSV, the CA dinucleotide in the 5′ end of U3 is not essential for appropriate integration. This result differs from the results seen with mutations in the U5 end, where the CA appears to be essential for proper integration in vivo. In addition, based on the structure of circular viral DNAs smaller than the full-length viral genome, our results suggest that there is little, if any, integrase-mediated autointegration of RSV linear DNA in vivo.

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