scholarly journals Structural changes inducted by follicle-stimulating hormone or dibutyryl cyclic AMP on presumptive Sertoli cells in culture.

1975 ◽  
Vol 72 (5) ◽  
pp. 1838-1842 ◽  
Author(s):  
P. S. Tung ◽  
J. H. Dorrington ◽  
I. B. Fritz
Keyword(s):  
Cyclic Amp ◽  
Sertoli Cells ◽  
Structural Changes ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Cyclic Amp ◽  
Cells In Culture ◽  
Stimulating Hormone

scholarly journals Follicle-Stimulating Hormone and Cyclic AMP Induce Transcription from the Human Urokinase Promoter in Primary Cultures of Mouse Sertoli Cells

1990 ◽  
Vol 4 (6) ◽  
pp. 940-946 ◽  
Author(s):  
Pellegrino Rossi ◽  
Paola Grimaldi ◽  
Francesco Blasi ◽  
Raffaele Geremia ◽  
Pasquale Verde
Keyword(s):  
Cyclic Amp ◽  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Primary Cultures ◽  
Stimulating Hormone

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

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